FastQCFastQC Report
Thu 23 Mar 2023
EGAF00000590614

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000590614
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences54358888
Sequences flagged as poor quality0
Sequence length90
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGGAACTATCTCGTA3325550.6117766794640832TruSeq Adapter, Index 20 (97% over 37bp)
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT2537030.46671852448490114No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT2093340.38509617783204103No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA1746040.3212059819913903No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1738560.31982994206945514No Hit
AGGAGGAGGACGGACGGACGGACGGACGGGGCCCCCCGAGCCACCTTCCC1633270.3004605245052106No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT1480630.27238047989502656No Hit
CGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAGGGACGCA1160970.21357500911350505No Hit
CGCCTCATTTGGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTT1062870.1955282823298372No Hit
GGGAGGAGGAGGACGGACGGACGGACGGACGGGGCCCCCCGAGCCACCTT1055040.19408785551315913No Hit
GGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAG1010550.1859033613785477No Hit
CCCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGTC986870.18154712804279588No Hit
CACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCAT982110.1806714662742917No Hit
TGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACGAGGA977280.17978292712683894No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG959330.17648079923930748No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG923200.16983423207627057No Hit
TGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGAC918280.16892913629874107No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA857060.15766694859541644No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC854160.15713345718183197No Hit
AGGAGGACGGACGGACGGACGGACGGGGCCCCCCGAGCCACCTTCCCCGC834190.15345972493035545No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG803380.14779183856741146No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC709610.13054166965299216No Hit
GGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACGAGGAG692920.12747133458653534No Hit
CCCCGTTCTCTGGGAACTCACCTCCCCGAAGCTCAGGGAGAGCCCTGTTA688960.1267428428631579No Hit
GGAGGTTCTAGCAGGGGAGCGCAGCTACTCGTATACCCTTGACCGAAGAC671530.12353637550495881No Hit
CCCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATC653390.12019929473170975No Hit
CCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGC648560.11931075558425698No Hit
GGACGGACGGACGGACGGACGGGGCCCCCCGAGCCACCTTCCCCGCCGGG565330.10399955201438264No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)