FastQCFastQC Report
Mon 3 Apr 2023
EGAF00000590626

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000590626
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences49110599
Sequences flagged as poor quality0
Sequence length90
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTCCTCCATCTCGTA5690961.1588048437364815TruSeq Adapter, Index 27 (97% over 39bp)
TGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGA5050021.0282953380389435No Hit
GGGGGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGC1709810.3481549878876452No Hit
GGGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCC1557400.3171209538698561No Hit
GGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAA1288560.26237920657412467No Hit
CCCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATC1078460.21959821748458005No Hit
GGCGGCCAAGCGTTCATAGCGACGTCGCTTTTTGATCCTTCGATGTCGGC1035530.21085672361683067No Hit
GTGGGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGT995090.2026222486107327No Hit
GACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAG868280.1768009386324121No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTCCTCCATCTCGTAT850110.1731011262965862TruSeq Adapter, Index 27 (97% over 39bp)
CAGGTGGGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGG700960.14273090010569817No Hit
GGCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTA644670.1312690158798511No Hit
CTCCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGG634670.1292327955519337No Hit
GGGGCCGAAACGATCTCAACCTATTCTCAAACTTTAAATGGGTAAGAAGC607930.12378794239508258No Hit
TGCCCTTCGTCCTGGGAAACGGGGCGCGGCCGGAAAGGCGGCCGCCCCCT542550.11047513389115861No Hit
GGGGCGAAGCTACCATCTGTGGGATTATGACTGAACGCCTCTAAGTCAGA513180.10449475478806519No Hit
GGGGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCA508310.10350311548836942No Hit
GGCGGAATCAGCGGGGAAAGAAGACCCTGTTGAGCTTGACTCTAGTCTGG498150.10143431563520534No Hit
GGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCA493080.10040195192895122No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)