FastQCFastQC Report
Fri 15 Sep 2023
EGAF00000590637

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000590637
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences47160874
Sequences flagged as poor quality0
Sequence length90
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTT2660970.5642325458175351No Hit
CCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTT2396320.5081161133697395No Hit
GGGAAGCTCATCAGTGGGGCCACGAGCTGAGTGCGTCCTGTCACTCCACT2016240.4275238834632284No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGG1709430.3624678372160788No Hit
AGCTTTTTTGAAAGTTAATCTCCTGGTTCACCGTCTGCTCGTACGCGGTT1592770.3377312303414903No Hit
AGCTTTAATGCGGTAGTTTATCACAGTTAAATTGCTAGCGCCGGCCGGCG1562630.33134033945172436No Hit
CGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGG1515410.3213278023643073No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGG1390850.2949160781032175No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGA1121440.23779033442001096No Hit
AGCTTAAGGTGTCGCCGCGAATGGCATGATCGTTCATGTCCTCCACCGGC1094310.23203768445851958No Hit
AGCTTTAACAACCACGTGCGCACGCTTGTGGCGCGCGAGGATATAATTAA1064360.22568708120209985No Hit
AGCTTAAAAGATGACACCGAACAGGGCGGGGATGGCGCAGGCGGCGGCAA1046030.2218003847850657No Hit
AGGGAAGCTCATCAGTGGGGCCACGAGCTGAGTGCGTCCTGTCACTCCAC1030630.21853496608226558No Hit
AGCTTTTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAA1012640.2147203633249036No Hit
AGCTTATCGATGATAAGCTGTCAAACATGAGAATTCTTGAAGACGAAAGG986270.20912886389679716No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA928510.19688142335954167No Hit
GATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGC922050.19551164382576963No Hit
AGCTTGCATGCCAGAGGCCAGGCCATCTACCACCTTTTGTTGGAAATTTG885310.18772128777766078No Hit
AGCTTATCGATGATAAGCTGTCAAACATGAGAATTCTTGGGGCCGGCGGG871800.18485662500656794No Hit
AGCTTGTGGAACTTTCGATCCCGCATCTCCTCGGGCTCCTCTCCCTCGCG862810.1829503838287645No Hit
GGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATT855220.18134099889667016No Hit
AGCTTGATCCCCGCCCTCCCGTAGAGGAGCTTCCACCGAATTTTAATATG852610.1807875740385982No Hit
CAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCT747350.15846822516478384No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC710890.1507372403658168Illumina Single End PCR Primer 1 (100% over 50bp)
CATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGCTGGC704440.14936958123379987No Hit
CGACCATCCCCGATAGAGGAGGACCGGTCTTCGGTCAAGGGTATACGAGT680810.14435907188658123No Hit
CAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTCT669220.14190152625246089No Hit
CGTCCCCCGACCGGCGACCGGCCGCCGCCGGGCGCATTTCCACCGCGGCG642080.13614675588921446No Hit
AGCTTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCT592000.12552778389984884No Hit
GGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTC554480.11757203651484491No Hit
AGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTG523780.11106240312679533No Hit
AGCTTGTAGAGTTACTTGAATTGCTCATATACTTAATTAAAAAATCCCAG510210.10818501794517209No Hit
AGCTTTAATGCGGTAGTTTATCACAGTTAAATTGCTAACGCAGTCAGGCG509390.10801114500125678No Hit
CAGAAGCAGGTCGTCTACGAATGGTTTAGCGCCAGGTTCCCCACGAACGT491290.10417321782458908No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)