FastQCFastQC Report
Thu 14 Sep 2023
EGAF00000590654

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000590654
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences45489695
Sequences flagged as poor quality0
Sequence length90
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGG7371691.6205186691183575No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT6212921.3657862511498484No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT5790001.27281574431308No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT4016580.88296481214042No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT3385090.744144360607386No Hit
CGCCTCATTTGGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTT3382990.7436827175913139No Hit
AGCTTTAATGCGGTAGTTTATCACAGTTAAATTGCTAGCGCCGGCCGGCG2782990.6117847129992848No Hit
AGCTTTTTTGAAAGTTAATCTCCTGGTTCACCGTCTGCTCGTACGCGGTT2742400.602861812988634No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCAAGGTATCTCGTA2666790.5862404661099618TruSeq Adapter, Index 1 (97% over 37bp)
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG2569970.5649565247689614No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA2227680.48971091144928536No Hit
AGCTTAAAAGATGACACCGAACAGGGCGGGGATGGCGCAGGCGGCGGCAA2031110.4464989268448601No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCAAGGTATCTCGTAT2012650.4424408649035787TruSeq Adapter, Index 1 (97% over 37bp)
AGCTTAAGGTGTCGCCGCGAATGGCATGATCGTTCATGTCCTCCACCGGC1996970.4389939303835737No Hit
AGCTTTAACAACCACGTGCGCACGCTTGTGGCGCGCGAGGATATAATTAA1951070.4289037330322835No Hit
AGCTTATCGATGATAAGCTGTCAAACATGAGAATTCTTGAAGACGAAAGG1942940.4271165150700615No Hit
AGCTTTTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAA1853730.40750548008730325No Hit
AGCTTGTGGAACTTTCGATCCCGCATCTCCTCGGGCTCCTCTCCCTCGCG1577920.34687416567642404No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG1444420.3175268596546976No Hit
CACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCAT1429620.3142733755414276No Hit
AGCTTGCATGCCAGAGGCCAGGCCATCTACCACCTTTTGTTGGAAATTTG1268850.2789313052109934No Hit
CTCATTTGGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTC1249320.2746380251615228No Hit
AGCTTGATCCCCGCCCTCCCGTAGAGGAGCTTCCACCGAATTTTAATATG1236760.2718769602653964No Hit
AGCTTATCGATGATAAGCTGTCAAACATGAGAATTCTTGGGGCCGGCGGG1203740.26461817341268173No Hit
CCCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATC1197260.26319367496308776No Hit
AGCTTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCT1179510.25929169232724025No Hit
AGCTTTAATGCGGTAGTTTATCACAGTTAAATTGCTAACGCAGTCAGGCG951820.2092385978846418No Hit
CCCGTTCTCTGGGAACTCACCTCCCCGAAGCTCAGGGAGAGCCCTGTTAG947950.2083878557550232No Hit
GCCTCATTTGGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTC937870.2061719692778771No Hit
AGCTTGTAGAGTTACTTGAATTGCTCATATACTTAATTAAAAAATCCCAG907160.19942098974284175No Hit
CCTCATTTGGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCT904720.19888460452416754No Hit
CTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAGG865730.19031343252576216No Hit
CCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGC858590.188743846271117No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC833970.18333163148269077No Hit
CCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG704230.15481088628974102No Hit
CCCCGTTCTCTGGGAACTCACCTCCCCGAAGCTCAGGGAGAGCCCTGTTA687710.15117929456330714No Hit
AGCTTGATCCCCGCCCTCCCGTAGAGGAGCCTCCACCGGCCGTGGAGACA682800.15009992922572904No Hit
CCCGTTCTCTGGGAACTCACCTCCCTGAAGCTCAGGGAGAGCCCTGTTAG645880.14198380534316618No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG643180.14139026432250204No Hit
TGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTG638150.14028451938400555No Hit
AGCTTATTTAAATAATATAAGCGCGCTGAGGGCGAGGCAGCGGCAGAAGG624890.1373695734825217No Hit
AGCTTATTTAAATAATATAAGCGCGCTGAGGCCGAGGCAGCGGCAGAAGG620580.13642210614953562No Hit
CTAGAGTATATACAAACGAAGCCTTCCATCCAGACATCATTTTGCTGCCG532800.11712542807772178No Hit
CTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC530700.11666378506164968No Hit
AGCTTGCCTGGTAGGCGTCCACATCCAACAGTGCGGGAATGTTACCACCC508120.11170002348883633No Hit
GCCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATA486880.10703083412627848No Hit
GGGAGGAGGAGGACGGACGGACGGACGGACGGGGCCCCCCGAGCCACCTT471240.10359269280657961No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)