FastQCFastQC Report
Thu 9 Feb 2023
EGAF00000590662

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000590662
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences103142351
Sequences flagged as poor quality0
Sequence length90
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTCGACAATCTCGTA12769561.2380520587513077TruSeq Adapter, Index 18 (97% over 38bp)
CCCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATC7719750.7484558888908786No Hit
CTCCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGG6588630.6387899767768528No Hit
GTCCCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAA3233110.31346095649884886No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT2992200.29010391667337504No Hit
CCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCG2794120.27089939029991666No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT2497580.24214883370265622No Hit
CCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGC2434610.2360436790896884No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT1692300.1640742123475545No Hit
CGCCTCATTTGGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTT1672650.16216907834493707No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTCGACAATCTCGTAT1598230.1549538074810802TruSeq Adapter, Index 18 (97% over 38bp)
TCCCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAAT1534440.1487691510929395No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1511120.14650819816973146No Hit
CCCCGTTCTCTGGGAACTCACCTCCCCGAAGCTCAGGGAGAGCCCTGTTA1146390.11114639029315902No Hit
CCCCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAAT1138750.11040566643667062No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)