FastQCFastQC Report
Tue 14 Feb 2023
EGAF00000590669

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000590669
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences98734095
Sequences flagged as poor quality0
Sequence length90
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTC14939161.5130700291525434No Hit
AGGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATC13854651.4032285402524831No Hit
ACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACT11311491.145651864231905No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGG7515310.7611666466381243No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGG6248820.6328938346981354No Hit
CATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTCC4856990.4919263198796727No Hit
AGGGAAGCTCATCAGTGGGGCCACGAGCTGAGTGCGTCCTGTCACTCCAC4675070.47350107376788125No Hit
CCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTT4570640.4629241803451989No Hit
GGGAAGCTCATCAGTGGGGCCACGAGCTGAGTGCGTCCTGTCACTCCACT4337200.43928087860632137No Hit
GGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCA4231490.42857434405004674No Hit
GGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCC3922160.39724474103905044No Hit
CCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTT3840410.38896492645220476No Hit
GGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCAT2591490.26247164163504005No Hit
ATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTCCG2456020.2487509507227468No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA2432190.24633739743094826No Hit
GATCAAGTGTAGTATCTGTTCTTATCAGTTTAATATCTGATACGTCCTCT2414070.24450216513353368No Hit
AAGATCAAGTGTAGTATCTGTTCTTATCAGTTTAATATCTGATACGTCCT2278580.23077944857852803No Hit
CAGGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTAT2160410.21881093861244183No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGA2153210.21808170723598572No Hit
CATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGCTGGC2091110.21179208661405163No Hit
CGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGG2084340.2111064065559116No Hit
CTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGA1886070.191025197526751No Hit
GGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATT1765250.1787882899012747No Hit
CGACCATCCCCGATAGAGGAGGACCGGTCTTCGGTCAAGGGTATACGAGT1687360.1708994243579181No Hit
GAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATT1527620.154720616014154No Hit
CGAAGCTGCGCGCTCGGTCGAAGAGGACGACCATCCCCGATAGAGGAGGA1286850.13033491622118984No Hit
AGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGT1192030.12073134412180514No Hit
GATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGC1155480.11702948206493412No Hit
CAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTCT1090390.11043702785749948No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1061910.10755251263507301Illumina Single End PCR Primer 1 (100% over 50bp)
TGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTCCGG1043690.10570715212409655No Hit
CGAGTAGCTGCGCTCCCCTGCTAGAACCTCCAAACAAGCTCTCAAGGTCC1041250.10546002371318643No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)