FastQCFastQC Report
Mon 18 Sep 2023
EGAF00000590674

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000590674
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences40231010
Sequences flagged as poor quality0
Sequence length90
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGA6717781.6698014790083568No Hit
CCCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATC3853450.9578307877430867No Hit
GGGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCC3755940.9335932654934589No Hit
GGGGGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGC2503260.6222215151943736No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAAGCACGATCTCGTA2224570.5529490808209886TruSeq Adapter, Index 7 (97% over 35bp)
GGCGGCCAAGCGTTCATAGCGACGTCGCTTTTTGATCCTTCGATGTCGGC2187990.5438565922158056No Hit
GGCGGAATCAGCGGGGAAAGAAGACCCTGTTGAGCTTGACTCTAGTCTGG2003940.49810830004019285No Hit
GTGGGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGT1937560.4816085899906565No Hit
GGCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTA1857350.46167123321040165No Hit
GTCCCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAA1693000.42081966125135806No Hit
TGCCCTTCGTCCTGGGAAACGGGGCGCGGCCGGAAAGGCGGCCGCCCCCT1572700.3909173545481458No Hit
GACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAG1550790.3854713068352No Hit
GGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAA1548530.38490955111492353No Hit
CTCCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGG1364500.3391662302288707No Hit
GGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCA1134250.28193425916973No Hit
TGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTC959360.23846281761258295No Hit
GGGGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCA940780.23384448961137191No Hit
GGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCT936360.23274583461861884No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT901170.2239988506378537No Hit
AGTCCCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAA898580.22335506863983778No Hit
GGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAA891660.2216350024520886No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC829640.20621903352662538No Hit
TGGGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTC784880.1950932874914152No Hit
GGGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCAC766690.19057189963662358No Hit
CGGGGAAAGAAGACCCTGTTGAGCTTGACTCTAGTCTGGCACGGTGAAGA723620.17986622756923082No Hit
CTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCT723220.17976680177803142No Hit
GTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGT705960.17547657888777835No Hit
CGCCTCATTTGGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTT688730.17119381293186525No Hit
AGCCGCTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGATCT687850.17097507619122662No Hit
CCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCG672480.1671546401643906No Hit
TCCCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAAT623150.15489295446472756No Hit
GGGGCCGAAACGATCTCAACCTATTCTCAAACTTTAAATGGGTAAGAAGC618440.15372221577335493No Hit
TGGCGGCCAAGCGTTCATAGCGACGTCGCTTTTTGATCCTTCGATGTCGG617870.1535805340208958No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT613030.15237748194738338No Hit
GGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA600820.14934250967102242No Hit
GGAGGTGTCAGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAG594970.14788840747473156No Hit
GCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGATG568490.14130642009733288No Hit
GTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGGGCATT562290.13976532033374256No Hit
GGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGA516740.12844320836091364No Hit
GGCACGGTGAAGAGACATGAGAGGTGTAGAATAAGTGGGAGGCCCCCGGC501320.1246103441101777No Hit
TGGGGCGAAGCTACCATCTGTGGGATTATGACTGAACGCCTCTAAGTCAG496770.12347937573528481No Hit
CGGCCAAGCGTTCATAGCGACGTCGCTTTTTGATCCTTCGATGTCGGCTC496040.12329792366634594No Hit
GGGGCGAAGCTACCATCTGTGGGATTATGACTGAACGCCTCTAAGTCAGA486930.12103350127178016No Hit
GGGGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTC483830.120262951389985No Hit
GGCGGGTGTTGACGCGATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAG481370.11965148277410882No Hit
CAGGTGGGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGG470500.11694958689826579No Hit
CCCCGTTCTCTGGGAACTCACCTCCCCGAAGCTCAGGGAGAGCCCTGTTA434560.10801617955900189No Hit
GCCCTTCGTCCTGGGAAACGGGGCGCGGCCGGAAAGGCGGCCGCCCCCTC426830.10609477614407395No Hit
CTGGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGC420740.10458101847306343No Hit
GGAGCGATTTGTCTGGTTAATTCCGATAACGAACGAGACTCTGGCATGCT416210.10345502138773052No Hit
CGGGTCATAAGCTTGCGTTGATTAAGTCCCTGCCCTTTGTACACACCGCC412550.10254527539825622No Hit
GCGGGTCATAAGCTTGCGTTGATTAAGTCCCTGCCCTTTGTACACACCGC411330.10224202673509812No Hit
CACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCAT406910.10114337174234503No Hit
CGGGTGTTGACGCGATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTG404590.10056670215338864No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)