FastQCFastQC Report
Wed 13 Sep 2023
EGAF00000590675

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000590675
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences40231010
Sequences flagged as poor quality0
Sequence length90
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACT3371630.8380674509538786No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC2185940.5433470350359089Illumina Single End PCR Primer 1 (100% over 50bp)
CAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCACGGGAGGTTTCTGTCCT1616770.40187159109353704No Hit
AGGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATC1468940.36512630431102777No Hit
CGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGG1421590.3533567762778016No Hit
CAGGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTAT1154780.28703728790303795No Hit
AGGATCGTGAGGCCCCGCTTTCACGGTCTGTATTCGTACTGAAAATCAAG1144470.2844745881348741No Hit
CCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTC1079960.26843969365919473No Hit
GAAGGATCGTGAGGCCCCGCTTTCACGGTCTGTATTCGTACTGAAAATCA1078940.26818615789163636No Hit
CAAAAGGTCAGAAGGATCGTGAGGCCCCGCTTTCACGGTCTGTATTCGTA991630.24648399331759258No Hit
GGATCGTGAGGCCCCGCTTTCACGGTCTGTATTCGTACTGAAAATCAAGA990400.2461782590096545No Hit
CGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAA871050.21651208856054074No Hit
AACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGT737630.18334861590598894No Hit
CCCCGCTTTCACGGTCTGTATTCGTACTGAAAATCAAGATCAAGCGAGCT697020.1732544124544723No Hit
CCTTCCGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTCCTCT686760.17070414091020833No Hit
AAGATCAAGTGTAGTATCTGTTCTTATCAGTTTAATATCTGATACGTCCT679420.16887967764169978No Hit
AGCGAGCTTTTGCCCTTCTGCTCCACGGGAGGTTTCTGTCCTCCCTGAGC652340.16214855157750202No Hit
GGTCTGTATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTG644900.16029923186119366No Hit
CCAAAAGGTCAGAAGGATCGTGAGGCCCCGCTTTCACGGTCTGTATTCGT637070.15835297199846587No Hit
CGCAGGGCCGGCGGACCCCGCCCCGGGCCCCTCGCGGGGACACCGGGGGG590150.14669032669077908No Hit
GAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCACGGGAGGTTTC573150.1424647305648056No Hit
AGCTGCTCTGCTACGTACGAAACCCCGACCCAGAAGCAGGTCGTCTACGA534050.13274585947506662No Hit
CAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCT525300.13057092029258027No Hit
CGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCACGGGA516370.1283512395040542No Hit
CCCAAAAGGTCAGAAGGATCGTGAGGCCCCGCTTTCACGGTCTGTATTCG510570.12690956553166327No Hit
CACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCT509270.1265864317102653No Hit
CGAGAAAGAGCTATCAATCTGTCAATCCTGTCCGTGTCCGGGCCGGGTGA494020.12279582342078908No Hit
CGACCATCCCCGATAGAGGAGGACCGGTCTTCGGTCAAGGGTATACGAGT484220.12035989153640439No Hit
TGAGGCCCCGCTTTCACGGTCTGTATTCGTACTGAAAATCAAGATCAAGC477980.11880884919369411No Hit
CCCGCTTTCACGGTCTGTATTCGTACTGAAAATCAAGATCAAGCGAGCTT469390.11667368032768752No Hit
AGGGAAGCTCATCAGTGGGGCCACGAGCTGAGTGCGTCCTGTCACTCCAC451140.11213737860421599No Hit
CGGACCCCGCCCCGGGCCCCTCGCGGGGACACCGGGGGGGCGCCGGGGGC447950.11134445791940097No Hit
CAGAAGCAGGTCGTCTACGAATGGTTTAGCGCCAGGTTCCCCACGAACGT444440.11047199660162645No Hit
AATCGCTCCACCAACTAAGAACGGCCATGCACCACCACCCACGGAATCGA435070.10814294744278106No Hit
CATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTCC432510.10750662237910508No Hit
CAGAAGGATCGTGAGGCCCCGCTTTCACGGTCTGTATTCGTACTGAAAAT421740.10482958295106187No Hit
AGATCAAGCGAGCTTTTGCCCTTCTGCTCCACGGGAGGTTTCTGTCCTCC411040.10216994303647857No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)