FastQCFastQC Report
Thu 16 Mar 2023
EGAF00000590686

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000590686
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences68085922
Sequences flagged as poor quality0
Sequence length90
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT2616930.3843569893934902No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCCAGAATCTCGTA2501670.36742837968765407TruSeq Adapter, Index 9 (97% over 37bp)
CCCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATC2024660.2973683752127202No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1880950.27626122181322593No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT1330570.1954251276791111No Hit
TGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGA1201450.17646085485924684No Hit
GTCCCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAA1162610.17075629819627028No Hit
GGCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTA922590.13550378299936955No Hit
CCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCG883480.12975957056144438No Hit
GGCGGCCAAGCGTTCATAGCGACGTCGCTTTTTGATCCTTCGATGTCGGC863770.12686469899019653No Hit
GGGCGGAGGAGAGTAGTCTGAATTGGGTTATGAGGTCCCCTGCGGGGTAC825950.1213099530325814No Hit
GCCTCATTTGGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTC742250.10901666279851509No Hit
GCAGTCCCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGG688200.10107816414676737No Hit
GGGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCC684520.10053767062154201No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)