FastQCFastQC Report
Fri 15 Sep 2023
EGAF00000590689

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000590689
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences49759336
Sequences flagged as poor quality0
Sequence length90
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCTTTAATGCGGTAGTTTATCACAGTTAAATTGCTAGCGCCGGCCGGCG8970721.8028214845953732No Hit
AGCTTTTTTGAAAGTTAATCTCCTGGTTCACCGTCTGCTCGTACGCGGTT8915141.7916517213975685No Hit
AGCTTAAGGTGTCGCCGCGAATGGCATGATCGTTCATGTCCTCCACCGGC8552641.7188010708181476No Hit
AGCTTAAAAGATGACACCGAACAGGGCGGGGATGGCGCAGGCGGCGGCAA8387491.6856113192507232No Hit
AGCTTTAACAACCACGTGCGCACGCTTGTGGCGCGCGAGGATATAATTAA6533711.313062135716602No Hit
AGCTTGTGGAACTTTCGATCCCGCATCTCCTCGGGCTCCTCTCCCTCGCG5757251.1570190566851615No Hit
AGCTTTTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAA5432601.091775018862792No Hit
AGCTTATCGATGATAAGCTGTCAAACATGAGAATTCTTGAAGACGAAAGG5232541.0515694984354294No Hit
AGCTTATCGATGATAAGCTGTCAAACATGAGAATTCTTGGGGCCGGCGGG4199500.8439622265055948No Hit
AGCTTGATCCCCGCCCTCCCGTAGAGGAGCTTCCACCGAATTTTAATATG4110250.8260258939146616No Hit
AGCTTGCATGCCAGAGGCCAGGCCATCTACCACCTTTTGTTGGAAATTTG3869200.777582723370746No Hit
AGCTTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCT3574310.7183194727518067No Hit
AGCTTTAATGCGGTAGTTTATCACAGTTAAATTGCTAACGCAGTCAGGCG3385330.6803406701407752No Hit
AGCTTGTAGAGTTACTTGAATTGCTCATATACTTAATTAAAAAATCCCAG3304850.6641668208755841No Hit
AGCTTGATCCCCGCCCTCCCGTAGAGGAGCCTCCACCGGCCGTGGAGACA2278320.4578678461465No Hit
AGCTTGCCTGGTAGGCGTCCACATCCAACAGTGCGGGAATGTTACCACCC2245820.45133640850834505No Hit
AGCTTATTTAAATAATATAAGCGCGCTGAGGCCGAGGCAGCGGCAGAAGG2013730.4046939050794408No Hit
AGCTTATTTAAATAATATAAGCGCGCTGAGGGCGAGGCAGCGGCAGAAGG1999770.40188840140471327No Hit
CTAGAGTATATACAAACGAAGCCTTCCATCCAGACATCATTTTGCTGCCG1912940.3844384097086826No Hit
GGATCGTGAGGCCCCGCTTTCACGGTCTGTATTCGTACTGAAAATCAAGA1418470.2850661029721136No Hit
GAAGGATCGTGAGGCCCCGCTTTCACGGTCTGTATTCGTACTGAAAATCA1405640.28248769235988197No Hit
AGGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATC1164190.23396413489118906No Hit
CCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTC1071690.21537465853644025No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1016190.20422097272359102Illumina Single End PCR Primer 1 (100% over 50bp)
AGCTTACTGTTAATCTTATTTTGCACTGCCTGGTTGGCCAGGTCCACCAC907850.18244817414766146No Hit
ACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACT863950.17362570915335365No Hit
GGTCTGTATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTG842390.16929285390785762No Hit
CAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCACGGGAGGTTTCTGTCCT779740.15670225181461425No Hit
AGCTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTT772540.155255287168623No Hit
CGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAA768290.15440117609286427No Hit
CGGACCCCGCCCCGGGCCCCTCGCGGGGACACCGGGGGGGCGCCGGGGGC709950.14267674311409623No Hit
CAAAAGGTCAGAAGGATCGTGAGGCCCCGCTTTCACGGTCTGTATTCGTA692860.13924221175298643No Hit
CCCGCTTTCACGGTCTGTATTCGTACTGAAAATCAAGATCAAGCGAGCTT675610.1357755256219657No Hit
CGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCACGGGA648100.13024691487040743No Hit
TGAGGCCCCGCTTTCACGGTCTGTATTCGTACTGAAAATCAAGATCAAGC632270.1270656023223461No Hit
AGGATCGTGAGGCCCCGCTTTCACGGTCTGTATTCGTACTGAAAATCAAG630930.12679630612434217No Hit
CGCAGGGCCGGCGGACCCCGCCCCGGGCCCCTCGCGGGGACACCGGGGGG613160.12322511699111097No Hit
CGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAA597920.12016237515709614No Hit
GGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCA586300.11782713499231583No Hit
CTTGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTT521680.1048406272945443No Hit
GCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACA518060.1041131256253098No Hit
AACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGT505030.1014945215506895No Hit
CCTTCCGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTCCTCT501190.1007228070728275No Hit
GAGTAGTGGTATTTCACCGGCGGCCCGCAGGGCCGGCGGACCCCGCCCCG498750.10023244683168601No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)