FastQCFastQC Report
Mon 18 Sep 2023
EGAF00000590705

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000590705
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences39704972
Sequences flagged as poor quality0
Sequence length90
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGG3828240.9641714392847324No Hit
GGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATT2858930.7200433235414446No Hit
CCTGAGCCCATGCTCGGGATATCTCAGGATATCTCAGGATATCTCAGGAT2788350.7022672122775958No Hit
CCTGACTGATATCCCGAGATATCCTGAGATATCCTGAGATATCCTGAGAT2407580.6063673839135311No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGG1962140.49417992285701645No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGG1772950.44653097853840573No Hit
CCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCA1490010.3752703817547082No Hit
CAATATGGTGACCTCCCGGGAGCGGGGGACCACCAGGTTGCCTAAGGAGG1193500.30059207698219764No Hit
AGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGT1159570.2920465477220334No Hit
CCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCCCGAAGCTGCGCGCT1086120.2735476050707201No Hit
AGGGAAGCTCATCAGTGGGGCCACGAGCTGAGTGCGTCCTGTCACTCCAC1072640.2701525642682735No Hit
CGACCATCCCCGATAGAGGAGGACCGGTCTTCGGTCAAGGGTATACGAGT997480.2512229450759971No Hit
CCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTT984700.24800420461195638No Hit
TCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTG944420.23785937942482366No Hit
CATCAATATGGTGACCTCCCGGGAGCGGGGGACCACCAGGTTGCCTAAGG893970.22515316217827835No Hit
CCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTT881340.22197220035818183No Hit
AAGATCAAGTGTAGTATCTGTTCTTATCAGTTTAATATCTGATACGTCCT859850.2165597799691182No Hit
GTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGAC818190.20606739125769943No Hit
ATCCTGAGATATCCTGAGATATCCTGAGATATCCTGAGATATCCCGAGAT802090.20201248347436188No Hit
GGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGG757390.1907544475790085No Hit
GCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCACC753520.1897797585652497No Hit
CCCGGGAGCGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCA745940.18787067775793923No Hit
GCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCG720760.1815289027278498No Hit
CGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGA704860.17752436646977107No Hit
AAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCACCAGGTT704600.1774588834869346No Hit
ATCCTGAGATATCCCGAGATATCCTGAGATATCCTGAGATATCCTGAGAT687370.1731193765858845No Hit
ATCCTGAGATATCCTGAGATATCCCGAGATATCCTGAGATATCCTGAGAT686800.17297581773889678No Hit
ATCCTGAGATATCCTGAGATATCCTGAGATATCCCGAGATATCCTGAGAT682920.17199861014887505No Hit
AGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGT601990.15161577245288071No Hit
CCTCCCGGGAGCGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGC597140.15039426296535355No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT587920.14807213565092048No Hit
ACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACT582960.14682292182450096No Hit
CTGACTGATATCCCGAGATATCCTGAGATATCCTGAGATATCCTGAGATA570770.14375277735997397No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC554900.13975579683068406No Hit
CCCGAAGCTGCGCGCTCGGTCGAAGAGGACGACCATCCCCGATAGAGGAG553580.13942334476397567No Hit
CATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGCTGGC540110.13603082253779197No Hit
TCAATATGGTGACCTCCCGGGAGCGGGGGACCACCAGGTTGCCTAAGGAG535520.13487479603310135No Hit
CGAAGCTGCGCGCTCGGTCGAAGAGGACGACCATCCCCGATAGAGGAGGA535390.1348420545416831No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA524310.13205147204234272No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC518620.13061840214872836Illumina Single End PCR Primer 1 (100% over 50bp)
ATCCCGAGATATCCTGAGATATCCTGAGATATCCTGAGATATCCTGAGAT508590.12809227015699695No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGA491610.12381572766252044No Hit
CTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGA487890.1228788172927058No Hit
CCGCTCCATGTGCGTCCCTCCCGAAGCTGCGCGCTCGGTCGAAGAGGACG456740.11503345223363966No Hit
CCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCCCGAAGCTGCGCGCTCG447150.11261813759747771No Hit
GGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTC446620.11248465305554176No Hit
CTCAGGATATCTCAGGATATCTCAGGATATCTCGGGATATCTCAGGATAT445600.1122277582767216No Hit
CCAGGGTTGATTCGGCTGATCTGGCTGGCTAGGCGGGTGTCCCCTTCCTC429180.10809225605296988No Hit
AGAGGACGACCATCCCCGATAGAGGAGGACCGGTCTTCGGTCAAGGGTAT428560.10793610432466745No Hit
CTCAGGATATCTCAGGATATCTCGGGATATCTCAGGATATCTCAGGATAT419540.10566434853549324No Hit
ATCTCAGGATATCTCAGGATATCTCAGGATATCTCGGGATATCTCAGGAT416710.10495159145308049No Hit
GCCGTGGACCCCGCCCTTCGGGGAGGGGCCCGGCGGATGCCTCCTTTGCC412020.1037703791857604No Hit
ATCTCGGGATATCTCAGGATATCTCAGGATATCTCAGGATATCTCAGGAT406210.10230708637699076No Hit
CGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGG403300.10157418068447448No Hit
CTCAGGATATCTCGGGATATCTCAGGATATCTCAGGATATCTCAGGATAT401910.10122409858392546No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)