FastQCFastQC Report
Thu 23 Feb 2023
EGAF00000590714

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000590714
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences99085823
Sequences flagged as poor quality0
Sequence length90
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATC19471371.965101505994455No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAAGATCTCGTA9222310.9307396074209325TruSeq Adapter, Index 23 (97% over 38bp)
GTCCCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAA8862750.8944518733017942No Hit
CCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCG7750620.7822128095963838No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT6315370.6373636317276186No Hit
CCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGC6123150.6179642873834736No Hit
CTCCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGG5354910.5404315004781259No Hit
TCCCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAAT4737470.4781178433568645No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT4053150.4090544819918386No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT4036220.40734586218252433No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG3822280.38575447872093666No Hit
CCCCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAAT3427560.34591830558847964No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT2830040.2856150268843203No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG2361440.2383226912289965No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA2105200.21246228130940586No Hit
CGCCTCATTTGGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTT2073470.20926000685284715No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC1824380.18412119360405374No Hit
CCCCGTTCTCTGGGAACTCACCTCCCCGAAGCTCAGGGAGAGCCCTGTTA1579680.15942543061886866No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAAGATCTCGTAT1566930.15813866732478976TruSeq Adapter, Index 23 (97% over 38bp)
CACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCAT1477230.1490859090911522No Hit
CCCGTTCTCTGGGAACTCACCTCCCCGAAGCTCAGGGAGAGCCCTGTTAG1448200.1461561256851043No Hit
GCCCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAAT1105160.11153563310464706No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)