FastQCFastQC Report
Sat 4 Mar 2023
EGAF00000590718

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000590718
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences70895248
Sequences flagged as poor quality0
Sequence length90
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCTCCAAATCTCGTA8263291.1655633110980865TruSeq Adapter, Index 6 (97% over 36bp)
CTCCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGG7762141.0948745111943188No Hit
CCCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATC4125870.5819670734489849No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT2305070.3251374478582824No Hit
GTCCCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAA2118340.29879858802384046No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT2026810.2858879906873307No Hit
GGGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCC1938940.2734936479804683No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCTCCAAATCTCGTAT1533350.21628388971853232TruSeq Adapter, Index 6 (97% over 36bp)
CCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCG1496570.21109595385010851No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT1461240.2061125450890587No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1348810.19025393634281385No Hit
CGCCTCATTTGGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTT1297260.18298264504272557No Hit
TGCCCTTCGTCCTGGGAAACGGGGCGCGGCCGGAAAGGCGGCCGCCCCCT1152420.1625525028137288No Hit
CCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGC1080850.15245732690010477No Hit
CAGGTGGGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGG957920.1351176597901174No Hit
GTGGGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGT874450.12334395106425187No Hit
GTTGTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGA856530.12081627812346464No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC812130.11455351704249628No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG769470.10853618849037668No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG767650.10827947170732796No Hit
CCTTCGTCCTGGGAAACGGGGCGCGGCCGGAAAGGCGGCCGCCCCCTCGC761140.10736121552180761No Hit
TCCCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAAT761140.10736121552180761No Hit
CCCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACC751460.1059958207636145No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)