FastQCFastQC Report
Mon 6 Mar 2023
EGAF00000590720

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000590720
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences85931756
Sequences flagged as poor quality0
Sequence length90
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGACAAATCTCGTA10457131.2169110101741665TruSeq Adapter, Index 3 (97% over 40bp)
CTCCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGG8542290.9940783707480619No Hit
CCCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATC7808110.9086408056178906No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT3505850.4079807236803121No Hit
GTCCCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAA3386800.3941267067788071No Hit
CCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCG3302030.3842619019678825No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT3222080.3749580073750617No Hit
CCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGC3178020.36983068285023757No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGACAAATCTCGTAT2524610.29379243687281337TruSeq Adapter, Index 3 (97% over 40bp)
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT2288490.26631481847060123No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT2107690.24527486672098261No Hit
TCCCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAAT1634190.19017300193423256No Hit
CCCGTTCTCTGGGAACTCACCTCCCCGAAGCTCAGGGAGAGCCCTGTTAG1618010.18829011244690497No Hit
CCCCGTTCTCTGGGAACTCACCTCCCCGAAGCTCAGGGAGAGCCCTGTTA1511560.17590237536865883No Hit
CGCCTCATTTGGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTT1509680.17568359710931544No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG1462590.17020366719842198No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA1115950.12986468006076823No Hit
CCCCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAAT1091770.1270508192570858No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG960670.11179452681032143No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC943870.10983948704597635No Hit
CACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCAT887910.10332734268807449No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)