FastQCFastQC Report
Wed 22 May 2024
EGAF00000590736

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000590736
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences125422570
Total Bases11.2 Gbp
Sequences flagged as poor quality0
Sequence length90
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATC12301260.9807851967951222No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT5633030.4491241090020719No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT5516600.4398410908020781No Hit
CCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCG5376620.4286804201189627No Hit
GTCCCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAA3952490.3151338710409139No Hit
GCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTT2979300.23754097846982405No Hit
GGGCGGAGGAGAGTAGTCTGAATTGGGTTATGAGGTCCCCTGCGGGGTAC2800020.2232469004581871No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTAACGTATCTCGTA2776720.22138918059165907TruSeq Adapter, Index 22 (97% over 37bp)
TCCCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAAT2628980.20960980148947675No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2596050.2069842772317614No Hit
CCCCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAAT2341560.1866936708440913No Hit
GCAGTCCCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGG1589440.12672679247443264No Hit
GCCTCATTTGGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTC1567280.12495996533957165No Hit
GGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAA1421080.1133033711556062No Hit
GCCCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAAT1325600.1056907062261601No Hit
AGTCCCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAA1300910.1037221610113714No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG1258420.10033441349511496No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)