FastQCFastQC Report
Sat 25 May 2024
EGAF00000590738

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000590738
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences102586345
Total Bases9.2 Gbp
Sequences flagged as poor quality0
Sequence length90
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACCAGACTATCTCGTA28231132.751938379323291TruSeq Adapter, Index 1 (97% over 37bp)
CCTGAGCCCATGCTCGGGATATCTCAGGATATCTCAGGATATCTCAGGAT25919332.5265867499227115No Hit
CCTGACTGATATCCCGAGATATCCTGAGATATCCTGAGATATCCTGAGAT23100782.251837708030245No Hit
ATCCTGAGATATCCTGAGATATCCTGAGATATCCTGAGATATCCCGAGAT7091170.6912391702813858No Hit
ATCCTGAGATATCCTGAGATATCCTGAGATATCCCGAGATATCCTGAGAT6101490.5947662917515971No Hit
ATCCTGAGATATCCTGAGATATCCCGAGATATCCTGAGATATCCTGAGAT5996180.5845007929661593No Hit
ATCCTGAGATATCCCGAGATATCCTGAGATATCCTGAGATATCCTGAGAT5731370.5586874159518989No Hit
CGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAGGGACGCA5270010.5137145689321517No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT4807010.4685818565813998No Hit
ATCCCGAGATATCCTGAGATATCCTGAGATATCCTGAGATATCCTGAGAT4545140.4430550674166235No Hit
CTGACTGATATCCCGAGATATCCTGAGATATCCTGAGATATCCTGAGATA4514190.4400380966882093No Hit
ATCTCAGGATATCTCAGGATATCTCAGGATATCTCGGGATATCTCAGGAT4386050.427547155520552No Hit
ATCTCAGGATATCTCAGGATATCTCGGGATATCTCAGGATATCTCAGGAT4063010.39605758446701655No Hit
ATCTCGGGATATCTCAGGATATCTCAGGATATCTCAGGATATCTCAGGAT3961510.38616348013958385No Hit
GGGCGGAGGAGAGTAGTCTGAATTGGGTTATGAGGTCCCCTGCGGGGTAC3826070.3729609432912343No Hit
CTCAGGATATCTCAGGATATCTCAGGATATCTCGGGATATCTCAGGATAT3711420.361784991949952No Hit
ATCTCAGGATATCTCGGGATATCTCAGGATATCTCAGGATATCTCAGGAT3683010.35901561752687455No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACCAGACTATCTCGTAT3600850.3510067543589744TruSeq Adapter, Index 1 (97% over 37bp)
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG3471060.33835497307170853No Hit
CTCAGGATATCTCAGGATATCTCGGGATATCTCAGGATATCTCAGGATAT3419540.3333328621855082No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC3157060.307746610915907No Hit
CTCAGGATATCTCGGGATATCTCAGGATATCTCAGGATATCTCAGGATAT3135790.30567323555586273No Hit
AGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTG3093240.30152551004717054No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT2990530.2915134562986916No Hit
ATCTCAGGATATCTCAGGATATCTCAGGATATCTCAGGATATCTCGGGAT2881060.2808424454541197No Hit
CCTAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACGATCTCGT2654940.2588005255475278No Hit
CTCAGGATATCTCAGGATATCTCAGGATATCTCAGGATATCTCGGGATAT2474570.24121826350280828No Hit
GCCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATA2423960.23628485837954358No Hit
CCTAAGCTTCTTACATGGCCCAGGTGCAGTACAGCATGTGGGTGACGCGA2382990.23229114947023408No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA2381270.23212348583039974No Hit
CCTTCACGCTTCATACGCGGGTCATAGTTGGCAAAGTACCAGGCATTTTT2351090.22918157382446955No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG2242020.2185495545240451No Hit
CACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCAT2187730.21325742719462323No Hit
GGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTG2143380.20893423973726719No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT2137190.20833084559158435No Hit
GGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAG2032750.1981501534146674No Hit
CCGGAGCCCATGCTCGGGATATCTCAGGATATCTCAGGATATCTCAGGAT2029440.1978274983868467No Hit
GGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAA1944770.18957396327942086No Hit
CTCGGGATATCTCAGGATATCTCAGGATATCTCAGGATATCTCAGGATAT1911000.18628210216476668No Hit
GCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAGGGACGC1892790.1845070121174509No Hit
CCTGCATTATGTCGTGATTGAGCGGGATGAAAAGTACATGGCGAGTTTTG1872820.18256035927588607No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA1830060.17839216320651644No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1776890.17320921220070762No Hit
CCTTAGCCCATGCTCGGGATATCTCAGGATATCTCAGGATATCTCAGGAT1752900.17087069434046023No Hit
GGAGGTTCTAGCAGGGGAGCGCAGCTACTCGTATACCCTTGACCGAAGAC1710780.1667648847417266No Hit
CTGAGCCCATGCTCGGGATATCTCAGGATATCTCAGGATATCTCAGGATA1598130.15578389112118188No Hit
TCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAGGGACGC1560700.15213525737757788No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGA1559350.15200366091608No Hit
GGCGGACTTTGGAGTGGGAAGCGGGGAATGTCTACGTGCGTATGCACGTG1435430.13992408053917896No Hit
CCCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATC1391680.1356593803980442No Hit
GCGCCTCATTTGGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGT1331970.12983891764542346No Hit
GTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGT1290760.12582181380962545No Hit
CCCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATA1211430.11808881581656896No Hit
AGGTCGGAAGAGCACACGTCTGAACTCCAGTCACACCAGACTATCTCGTA1193980.11638780970313349TruSeq Adapter, Index 5 (97% over 35bp)
ACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATA1182300.11524925661402596No Hit
CAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTT1175990.11463416500509886No Hit
CCGGACTGATATCCCGAGATATCCTGAGATATCCTGAGATATCCTGAGAT1164080.11347319177810652No Hit
GGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAA1161480.11321974674114767No Hit
GCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGG1152030.11229857151066255No Hit
GCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTT1144950.11160842117925147No Hit
CCCGAGATATCCTGAGATATCCTGAGATATCCTGAGATATCCTGAGATAT1128220.10997759984528155No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)