FastQCFastQC Report
Thu 23 Feb 2023
EGAF00000590741

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000590741
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences93067392
Sequences flagged as poor quality0
Sequence length90
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGAGCCCATGCTCGGGATATCTCAGGATATCTCAGGATATCTCAGGAT10360821.113259948231922No Hit
CCTGACTGATATCCCGAGATATCCTGAGATATCCTGAGATATCCTGAGAT9306150.9999366910378233No Hit
CGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGG7656440.8226769693943932No Hit
GGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATT5164700.5549419500226245No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC3979820.42762775602436565Illumina Single End PCR Primer 1 (100% over 50bp)
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGG3124530.3357276842999963No Hit
AGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGT2767690.29738557624995016No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGG2750820.2955729112942157No Hit
ATCCTGAGATATCCTGAGATATCCTGAGATATCCTGAGATATCCCGAGAT2446600.262884770640183No Hit
CCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCA2217060.23822092274810922No Hit
ATCCTGAGATATCCTGAGATATCCTGAGATATCCCGAGATATCCTGAGAT2075190.22297713037881195No Hit
ATCCTGAGATATCCTGAGATATCCCGAGATATCCTGAGATATCCTGAGAT2043630.21958603932943557No Hit
ATCCTGAGATATCCCGAGATATCCTGAGATATCCTGAGATATCCTGAGAT1985650.21335614518992863No Hit
CCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCCCGAAGCTGCGCGCT1901600.20432505511704893No Hit
CTGACTGATATCCCGAGATATCCTGAGATATCCTGAGATATCCTGAGATA1899130.2040596560393569No Hit
AGGGAAGCTCATCAGTGGGGCCACGAGCTGAGTGCGTCCTGTCACTCCAC1817720.19531223137745174No Hit
TCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTG1776260.1908573950369212No Hit
CAATATGGTGACCTCCCGGGAGCGGGGGACCACCAGGTTGCCTAAGGAGG1671030.17955053473508745No Hit
ATCCCGAGATATCCTGAGATATCCTGAGATATCCTGAGATATCCTGAGAT1631470.17529985153124308No Hit
CGACCATCCCCGATAGAGGAGGACCGGTCTTCGGTCAAGGGTATACGAGT1439340.15465567145150044No Hit
CCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTT1431060.15376599357162604No Hit
ATCTCAGGATATCTCAGGATATCTCAGGATATCTCGGGATATCTCAGGAT1410200.15152460702885065No Hit
CATCAATATGGTGACCTCCCGGGAGCGGGGGACCACCAGGTTGCCTAAGG1379960.14827534868496153No Hit
GTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGAC1315490.14134811041014236No Hit
CTCAGGATATCTCAGGATATCTCAGGATATCTCGGGATATCTCAGGATAT1313920.141179415449828No Hit
ATCTCAGGATATCTCAGGATATCTCGGGATATCTCAGGATATCTCAGGAT1308020.14054546623590783No Hit
ATCTCGGGATATCTCAGGATATCTCAGGATATCTCAGGATATCTCAGGAT1306070.14033594064825627No Hit
CCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTT1281600.1377066631457772No Hit
GCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCG1281190.1376626090478607No Hit
GGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGG1278990.137426221205382No Hit
AGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGT1263770.13579084713150658No Hit
GCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCACC1234290.13262325004229195No Hit
CTCAGGATATCTCAGGATATCTCGGGATATCTCAGGATATCTCAGGATAT1203450.1293095222868177No Hit
ATCTCAGGATATCTCGGGATATCTCAGGATATCTCAGGATATCTCAGGAT1195840.12849183524988No Hit
CTCAGGATATCTCGGGATATCTCAGGATATCTCAGGATATCTCAGGATAT1130210.12143995611266296No Hit
AAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCACCAGGTT1129360.12134862444625072No Hit
CGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGA1125070.12088766815341727No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT1118980.12023330362582847No Hit
CATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGCTGGC1110270.11929742266765142No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC1107270.11897507560972591No Hit
AAGATCAAGTGTAGTATCTGTTCTTATCAGTTTAATATCTGATACGTCCT1074420.11544537532544158No Hit
ACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACT1063490.11427095754439964No Hit
CCCGGGAGCGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCA1052610.11310191221432313No Hit
CCCGAAGCTGCGCGCTCGGTCGAAGAGGACGACCATCCCCGATAGAGGAG985890.10593291364605983No Hit
ATCTCAGGATATCTCAGGATATCTCAGGATATCTCAGGATATCTCGGGAT942290.10124813640420911No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)