FastQCFastQC Report
Mon 6 Mar 2023
EGAF00000590742

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000590742
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences75198479
Sequences flagged as poor quality0
Sequence length90
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAGCGCTATCTCGTA12239081.627570153380363TruSeq Adapter, Index 5 (97% over 37bp)
CCTGAGCCCATGCTCGGGATATCTCAGGATATCTCAGGATATCTCAGGAT11322731.5057126354909385No Hit
CCTGACTGATATCCCGAGATATCCTGAGATATCCTGAGATATCCTGAGAT10245821.3625036219150124No Hit
CCTTAGCCCATGCTCGGGATATCTCAGGATATCTCAGGATATCTCAGGAT3697630.4917160625017429No Hit
CCTTACTGATATCCCGAGATATCCTGAGATATCCTGAGATATCCTGAGAT3363260.447251067405233No Hit
ATCCTGAGATATCCTGAGATATCCTGAGATATCCTGAGATATCCCGAGAT3171290.4217226255334234No Hit
ATCCTGAGATATCCTGAGATATCCTGAGATATCCCGAGATATCCTGAGAT2707930.3601043579618146No Hit
ATCCTGAGATATCCTGAGATATCCCGAGATATCCTGAGATATCCTGAGAT2636650.35062544283641695No Hit
ATCCTGAGATATCCCGAGATATCCTGAGATATCCTGAGATATCCTGAGAT2563310.340872586000044No Hit
CTGACTGATATCCCGAGATATCCTGAGATATCCTGAGATATCCTGAGATA2293960.3050540423829583No Hit
ATCCCGAGATATCCTGAGATATCCTGAGATATCCTGAGATATCCTGAGAT2094990.278594730619485No Hit
ATCTCAGGATATCTCAGGATATCTCAGGATATCTCGGGATATCTCAGGAT1917090.25493733722991924No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA1841340.24486399518798777No Hit
CGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAGGGACGCA1833270.24379083518431272No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA1778990.2365726040815267No Hit
ATCTCAGGATATCTCAGGATATCTCGGGATATCTCAGGATATCTCAGGAT1768480.2351749694299003No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAGCGCTATCTCGTAT1748730.2325485865212779TruSeq Adapter, Index 5 (97% over 37bp)
ATCTCGGGATATCTCAGGATATCTCAGGATATCTCAGGATATCTCAGGAT1708290.22717081817572402No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG1707390.2270511349039387No Hit
GGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTG1693820.2252465771282422No Hit
GGGCGGAGGAGAGTAGTCTGAATTGGGTTATGAGGTCCCCTGCGGGGTAC1661000.22088212715047068No Hit
AGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTG1640590.21816797650920572No Hit
ATCTCAGGATATCTCGGGATATCTCAGGATATCTCAGGATATCTCAGGAT1584670.2107316558889442No Hit
CTCAGGATATCTCAGGATATCTCAGGATATCTCGGGATATCTCAGGATAT1574850.20942577841235327No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT1551100.20626746985135166No Hit
AGAGCGGAAGAGCACACGTCTGAACTCCAGTCACCCAGCGCTATCTCGTA1520150.20215169511606743TruSeq Adapter, Index 23 (97% over 36bp)
GGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAG1454920.19347731753989333No Hit
CTCAGGATATCTCAGGATATCTCGGGATATCTCAGGATATCTCAGGATAT1448420.1926129383547771No Hit
CTCAGGATATCTCGGGATATCTCAGGATATCTCAGGATATCTCAGGATAT1316280.17504077442842958No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC1310330.17424953502051552No Hit
ATCTCAGGATATCTCAGGATATCTCAGGATATCTCAGGATATCTCGGGAT1281740.17044759642013504No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGA1212500.16123996337745075No Hit
GCCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATA1195880.15902981229181512No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT1113690.1481000699495531No Hit
GGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAA1094320.1455242199779067No Hit
CTCAGGATATCTCAGGATATCTCAGGATATCTCAGGATATCTCGGGATAT1086750.14451755068077907No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1063660.14144700985242004No Hit
CACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCAT1038340.13807992047285958No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT920780.12244662554943431No Hit
CTGAGCCCATGCTCGGGATATCTCAGGATATCTCAGGATATCTCAGGATA915630.12176177127199608No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG892930.11874309319474399No Hit
GCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGG798520.10618831798446349No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT780850.10383853641507829No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)