FastQCFastQC Report
Wed 22 May 2024
EGAF00000590744

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000590744
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences92674256
Total Bases8.3 Gbp
Sequences flagged as poor quality0
Sequence length90
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGAGCCCATGCTCGGGATATCTCAGGATATCTCAGGATATCTCAGGAT28450823.069980944869954No Hit
CCTGACTGATATCCCGAGATATCCTGAGATATCCTGAGATATCCTGAGAT25356382.7360759173507687No Hit
ATCCTGAGATATCCTGAGATATCCTGAGATATCCTGAGATATCCCGAGAT6304200.6802536402342415No Hit
ATCCTGAGATATCCTGAGATATCCTGAGATATCCCGAGATATCCTGAGAT5410320.5837996692414774No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAACCTATCTCGTA5403660.5830810230621112TruSeq Adapter, Index 19 (97% over 38bp)
ATCCTGAGATATCCTGAGATATCCCGAGATATCCTGAGATATCCTGAGAT5307400.5726941039591404No Hit
ATCCTGAGATATCCCGAGATATCCTGAGATATCCTGAGATATCCTGAGAT5115550.5519925619904626No Hit
CTGACTGATATCCCGAGATATCCTGAGATATCCTGAGATATCCTGAGATA5079520.5481047509029908No Hit
CGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAGGGACGCA4814620.51952076097595No Hit
ATCCCGAGATATCCTGAGATATCCTGAGATATCCTGAGATATCCTGAGAT4191340.4522658374511256No Hit
ATCTCAGGATATCTCAGGATATCTCAGGATATCTCGGGATATCTCAGGAT3773870.40721880734602284No Hit
GGGCGGAGGAGAGTAGTCTGAATTGGGTTATGAGGTCCCCTGCGGGGTAC3581600.38647194534801554No Hit
ATCTCAGGATATCTCAGGATATCTCGGGATATCTCAGGATATCTCAGGAT3498750.3775320300386334No Hit
ATCTCGGGATATCTCAGGATATCTCAGGATATCTCAGGATATCTCAGGAT3382220.3649578799963606No Hit
CTCAGGATATCTCAGGATATCTCAGGATATCTCGGGATATCTCAGGATAT3296780.3557384911727805No Hit
ATCTCAGGATATCTCGGGATATCTCAGGATATCTCAGGATATCTCAGGAT3132580.3380205178016212No Hit
CTCAGGATATCTCAGGATATCTCGGGATATCTCAGGATATCTCAGGATAT3026610.32658584278248753No Hit
CTCAGGATATCTCGGGATATCTCAGGATATCTCAGGATATCTCAGGATAT2748510.2965775090765228No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA2715910.29305981156190775No Hit
GGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTG2676060.2887598040172019No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC2604150.28100036756701885No Hit
ATCTCAGGATATCTCAGGATATCTCAGGATATCTCAGGATATCTCGGGAT2517540.2716547300903069No Hit
AGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTG2515920.27147992426289347No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA2429630.26216881633233724No Hit
CTCAGGATATCTCAGGATATCTCAGGATATCTCAGGATATCTCGGGATAT2231920.2408349520496825No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG2081420.22459527487331543No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT2048600.22105383829571826No Hit
GGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAA2035460.21963596880669858No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT2002700.21610100651900566No Hit
CTGAGCCCATGCTCGGGATATCTCAGGATATCTCAGGATATCTCAGGATA1954400.21088920314612505No Hit
CTCGGGATATCTCAGGATATCTCAGGATATCTCAGGATATCTCAGGATAT1887920.20371568993227201No Hit
GGAGGTTCTAGCAGGGGAGCGCAGCTACTCGTATACCCTTGACCGAAGAC1733020.18700123149626363No Hit
GGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAG1532310.16534365271839896No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGA1459380.15747415333984444No Hit
CCTAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACGATCTCGT1453550.15684506817081972No Hit
GCCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATA1425860.15385718338003165No Hit
GCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAGGGACGC1403740.15147032850201678No Hit
CCTAAGCTTCTTACATGGCCCAGGTGCAGTACAGCATGTGGGTGACGCGA1395410.1505714812536504No Hit
CCTTCACGCTTCATACGCGGGTCATAGTTGGCAAAGTACCAGGCATTTTT1324300.14289836866885663No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT1316200.14202433953178972No Hit
GGGGTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCG1282760.13841600195851586No Hit
CCCGAGATATCCTGAGATATCCTGAGATATCCTGAGATATCCTGAGATAT1196270.12908331306161228No Hit
CCTGCATTATGTCGTGATTGAGCGGGATGAAAAGTACATGGCGAGTTTTG1154940.12462360636593618No Hit
CCTGAATTCAGATCTCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGG1154740.12460202539958885No Hit
CACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCAT1140150.12302769390455101No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1115080.12032251977291297No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG1074320.11592431883132678No Hit
TCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAGGGACGC1042480.1124886289888316No Hit
CAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTT936080.10100755489205115No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)