FastQCFastQC Report
Mon 6 Mar 2023
EGAF00000590748

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000590748
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences81885243
Sequences flagged as poor quality0
Sequence length90
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAGATCTATCTCGTA14661951.7905485118973146TruSeq Adapter, Index 5 (97% over 37bp)
CCTGAGCCCATGCTCGGGATATCTCAGGATATCTCAGGATATCTCAGGAT12995301.587013669850134No Hit
CCTGACTGATATCCCGAGATATCCTGAGATATCCTGAGATATCCTGAGAT11314501.381750799713692No Hit
CCTTAGCCCATGCTCGGGATATCTCAGGATATCTCAGGATATCTCAGGAT4303390.5255391377418273No Hit
CCTTACTGATATCCCGAGATATCCTGAGATATCCTGAGATATCCTGAGAT3774960.46100614246207955No Hit
ATCCTGAGATATCCTGAGATATCCTGAGATATCCTGAGATATCCCGAGAT3517180.4295255007059086No Hit
GGGCGGAGGAGAGTAGTCTGAATTGGGTTATGAGGTCCCCTGCGGGGTAC3297170.40265741166573815No Hit
CTGACTGATATCCCGAGATATCCTGAGATATCCTGAGATATCCTGAGATA3100830.378679953358629No Hit
ATCCTGAGATATCCTGAGATATCCTGAGATATCCCGAGATATCCTGAGAT2992880.3654968698083976No Hit
ATCCTGAGATATCCTGAGATATCCCGAGATATCCTGAGATATCCTGAGAT2914020.35586631891658427No Hit
ATCCTGAGATATCCCGAGATATCCTGAGATATCCTGAGATATCCTGAGAT2759450.3369899018312738No Hit
CGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAGGGACGCA2292610.2799784083195552No Hit
ATCCCGAGATATCCTGAGATATCCTGAGATATCCTGAGATATCCTGAGAT2238000.27330931899414407No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC2131810.26034117038646387No Hit
CTCAGGATATCTCAGGATATCTCAGGATATCTCGGGATATCTCAGGATAT2000160.24426379243937765No Hit
ATCTCAGGATATCTCAGGATATCTCAGGATATCTCGGGATATCTCAGGAT1965300.24000661511134552No Hit
AGAGCGGAAGAGCACACGTCTGAACTCCAGTCACAGAGATCTATCTCGTA1866210.22790553360145732TruSeq Adapter, Index 13 (97% over 35bp)
CTCAGGATATCTCAGGATATCTCGGGATATCTCAGGATATCTCAGGATAT1812660.2213658937300827No Hit
ATCTCAGGATATCTCAGGATATCTCGGGATATCTCAGGATATCTCAGGAT1784180.21788785556879886No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAGATCTATCTCGTAT1718300.20984244987830103TruSeq Adapter, Index 5 (97% over 37bp)
ATCTCGGGATATCTCAGGATATCTCAGGATATCTCAGGATATCTCAGGAT1700030.2076112786280673No Hit
CTCAGGATATCTCGGGATATCTCAGGATATCTCAGGATATCTCAGGATAT1619760.19780853553796013No Hit
ATCTCAGGATATCTCGGGATATCTCAGGATATCTCAGGATATCTCAGGAT1607540.19631620315274634No Hit
GGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAA1600940.19551019711817916No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1495410.18262264911395573No Hit
CTCAGGATATCTCAGGATATCTCAGGATATCTCAGGATATCTCGGGATAT1356380.16564400987367162No Hit
ATCTCAGGATATCTCAGGATATCTCAGGATATCTCAGGATATCTCGGGAT1317600.16090811380995718No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG1314750.1605600657495759No Hit
AGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTG1278160.15609161714278602No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT1265030.15448815362250315No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA1118820.1366326775143111No Hit
CTGAGCCCATGCTCGGGATATCTCAGGATATCTCAGGATATCTCAGGATA1097080.1339777424852974No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA1080630.13196883350520192No Hit
GGAGGTTCTAGCAGGGGAGCGCAGCTACTCGTATACCCTTGACCGAAGAC1017470.12425559999864688No Hit
GGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTG961140.11737646061574245No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT872020.1064929367065565No Hit
GGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAG844310.10310893258263884No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)