FastQCFastQC Report
Mon 6 Mar 2023
EGAF00000590749

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000590749
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences81885243
Sequences flagged as poor quality0
Sequence length90
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGAGCCCATGCTCGGGATATCTCAGGATATCTCAGGATATCTCAGGAT18060202.2055500281045757No Hit
CCTGACTGATATCCCGAGATATCCTGAGATATCCTGAGATATCCTGAGAT15819411.931899988377637No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC14752861.8016506344128453Illumina Single End PCR Primer 1 (100% over 50bp)
CGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGG4760350.5813440646442242No Hit
GGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATT3737740.4564607569156264No Hit
ATCCTGAGATATCCTGAGATATCCTGAGATATCCTGAGATATCCCGAGAT3521960.4301092444703376No Hit
CTGACTGATATCCCGAGATATCCTGAGATATCCTGAGATATCCTGAGATA3339550.407832947384671No Hit
ATCCTGAGATATCCTGAGATATCCTGAGATATCCCGAGATATCCTGAGAT2989220.3650499028255922No Hit
ATCCTGAGATATCCTGAGATATCCCGAGATATCCTGAGATATCCTGAGAT2894120.3534360886002378No Hit
ATCCTGAGATATCCCGAGATATCCTGAGATATCCTGAGATATCCTGAGAT2793650.3411664785558492No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGG2398850.2929526630335578No Hit
ATCCCGAGATATCCTGAGATATCCTGAGATATCCTGAGATATCCTGAGAT2377720.2903722225016783No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGG2295350.280313022946027No Hit
CTCAGGATATCTCAGGATATCTCAGGATATCTCGGGATATCTCAGGATAT1973240.2409762647953551No Hit
ATCTCAGGATATCTCAGGATATCTCAGGATATCTCGGGATATCTCAGGAT1927440.23538307140396467No Hit
AGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGT1880080.2295993674953129No Hit
CTCAGGATATCTCAGGATATCTCGGGATATCTCAGGATATCTCAGGATAT1793600.21903824599995386No Hit
ATCTCAGGATATCTCAGGATATCTCGGGATATCTCAGGATATCTCAGGAT1766460.21572385148811243No Hit
ATCTCGGGATATCTCAGGATATCTCAGGATATCTCAGGATATCTCAGGAT1683230.20555962690371452No Hit
CTCAGGATATCTCGGGATATCTCAGGATATCTCAGGATATCTCAGGATAT1640100.2002924995899444No Hit
ATCTCAGGATATCTCGGGATATCTCAGGATATCTCAGGATATCTCAGGAT1588290.19396535221859204No Hit
CCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCCCGAAGCTGCGCGCT1504160.1836912177203895No Hit
CTCAGGATATCTCAGGATATCTCAGGATATCTCAGGATATCTCGGGATAT1360220.166112958839238No Hit
CCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCA1335060.16304036613776673No Hit
AGGGAAGCTCATCAGTGGGGCCACGAGCTGAGTGCGTCCTGTCACTCCAC1312770.16031826393920573No Hit
ATCTCAGGATATCTCAGGATATCTCAGGATATCTCAGGATATCTCGGGAT1299660.15871724286145183No Hit
CTGAGCCCATGCTCGGGATATCTCAGGATATCTCAGGATATCTCAGGATA1204590.14710709229989097No Hit
CCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTT1014100.12384404843251183No Hit
CCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTT982970.12004238663613663No Hit
TCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTG966660.11805057475374409No Hit
CTCGGGATATCTCAGGATATCTCAGGATATCTCAGGATATCTCAGGATAT962810.11758040456691324No Hit
CGACCATCCCCGATAGAGGAGGACCGGTCTTCGGTCAAGGGTATACGAGT932180.11383980383376281No Hit
AAGATCAAGTGTAGTATCTGTTCTTATCAGTTTAATATCTGATACGTCCT925730.11305211611816307No Hit
CAATATGGTGACCTCCCGGGAGCGGGGGACCACCAGGTTGCCTAAGGAGG856230.10456462832991777No Hit
CGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGA855140.10443151521208771No Hit
GGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGG835300.1020086122233282No Hit
CCCGAAGCTGCGCGCTCGGTCGAAGAGGACGACCATCCCCGATAGAGGAG823890.10061519876053858No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)