FastQCFastQC Report
Fri 10 Mar 2023
EGAF00000590763

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000590763
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences84051337
Sequences flagged as poor quality0
Sequence length90
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCTTTAATGCGGTAGTTTATCACAGTTAAATTGCTAGCGCCGGCCGGCG20768892.4709767555511934No Hit
AGCTTTTTTGAAAGTTAATCTCCTGGTTCACCGTCTGCTCGTACGCGGTT20669022.4590947315924314No Hit
AGCTTAAAAGATGACACCGAACAGGGCGGGGATGGCGCAGGCGGCGGCAA15936551.8960495536198312No Hit
AGCTTAAGGTGTCGCCGCGAATGGCATGATCGTTCATGTCCTCCACCGGC15721801.8704996923487367No Hit
AGCTTTAACAACCACGTGCGCACGCTTGTGGCGCGCGAGGATATAATTAA13357051.5891537810992822No Hit
AGCTTTTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAA12429081.478748636681413No Hit
AGCTTATCGATGATAAGCTGTCAAACATGAGAATTCTTGAAGACGAAAGG11796591.403498197774058No Hit
AGCTTGTGGAACTTTCGATCCCGCATCTCCTCGGGCTCCTCTCCCTCGCG11566831.376162523149394No Hit
AGCTTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCT7438180.8849567735014138No Hit
AGCTTTAATGCGGTAGTTTATCACAGTTAAATTGCTAACGCAGTCAGGCG6868520.8171815279987752No Hit
AGCTTGTAGAGTTACTTGAATTGCTCATATACTTAATTAAAAAATCCCAG6333370.753512106535557No Hit
AGCTTATCGATGATAAGCTGTCAAACATGAGAATTCTTGGGGCCGGCGGG5406580.6432473525079083No Hit
AGCTTGATCCCCGCCCTCCCGTAGAGGAGCTTCCACCGAATTTTAATATG5281490.6283647813954465No Hit
AGCTTGCCTGGTAGGCGTCCACATCCAACAGTGCGGGAATGTTACCACCC5225050.621649837646247No Hit
CCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTT5178530.6161151249741571No Hit
AGCTTGCATGCCAGAGGCCAGGCCATCTACCACCTTTTGTTGGAAATTTG4955400.5895682539826821No Hit
AGCTTGATCCCCGCCCTCCCGTAGAGGAGCCTCCACCGGCCGTGGAGACA4887750.5815196015263863No Hit
CCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTT4160200.49495940796277876No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGG3453880.4109250516740739No Hit
CTAGAGTATATACAAACGAAGCCTTCCATCCAGACATCATTTTGCTGCCG3397410.4042065386776655No Hit
GGGAAGCTCATCAGTGGGGCCACGAGCTGAGTGCGTCCTGTCACTCCACT3234470.3848207673365148No Hit
AGCTTACTGTTAATCTTATTTTGCACTGCCTGGTTGGCCAGGTCCACCAC3022260.3595731023291159No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGG2856990.33991011945473276No Hit
AGCTTATTTAAATAATATAAGCGCGCTGAGGCCGAGGCAGCGGCAGAAGG2669740.31763206812522204No Hit
AGCTTATTTAAATAATATAAGCGCGCTGAGGGCGAGGCAGCGGCAGAAGG2657480.3161734357658106No Hit
CGTCCCCCGACCGGCGACCGGCCGCCGCCGGGCGCATTTCCACCGCGGCG2410850.2868306544606185No Hit
AGGGAAGCTCATCAGTGGGGCCACGAGCTGAGTGCGTCCTGTCACTCCAC2335350.2778480489846342No Hit
CGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAA2095110.24926551733495925No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA1704580.202802246917262No Hit
AGCTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTT1480160.17610189829579986No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGA1468060.17466230192150306No Hit
CGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGG1306580.15545023394452367No Hit
CAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTCT1276690.15189407397528965No Hit
CATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGCTGGC1211730.14416546401873417No Hit
CGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGA1144410.13615607328173734No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1134870.1350210526692752Illumina Single End PCR Primer 1 (100% over 50bp)
AGGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATC1100560.13093902361124846No Hit
CCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCCCGAAGCTGCGCGCT1097210.13054045767291006No Hit
AATATGGTGACCTCCCGGGAGCGGGGGACCACCAGGTTGCCTAAGGAGGG1093770.13013118399294468No Hit
GGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATT1073410.1277088548871031No Hit
CATCAATATGGTGACCTCCCGGGAGCGGGGGACCACCAGGTTGCCTAAGG1063350.1265119673230183No Hit
CAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCT1045760.12441919870947442No Hit
CTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGA1022670.12167206810761381No Hit
AGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGT979790.11657042409688259No Hit
CCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTC965980.11492738063167275No Hit
ATCAATATGGTGACCTCCCGGGAGCGGGGGACCACCAGGTTGCCTAAGGA933610.11107616289316137No Hit
ACCGTCCCCCGACCGGCGACCGGCCGCCGCCGGGCGCATTTCCACCGCGG923120.1098281161190809No Hit
TCGAGTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGA916580.1090500202275188No Hit
CGACCATCCCCGATAGAGGAGGACCGGTCTTCGGTCAAGGGTATACGAGT880940.10480975454322636No Hit
GATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGG876260.1042529519786223No Hit
CACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCACCA853150.10150344187862236No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)