FastQCFastQC Report
Fri 15 Sep 2023
EGAF00000590790

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000590790
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences45846872
Sequences flagged as poor quality0
Sequence length90
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGATATGGATCTCGTA11870692.589203904685144TruSeq Adapter, Index 11 (97% over 38bp)
AGGAGGAGGACGGACGGACGGACGGACGGGGCCCCCCGAGCCACCTTCCC3767940.8218532335204898No Hit
GGGAGGAGGAGGACGGACGGACGGACGGACGGGGCCCCCCGAGCCACCTT2797500.6101833948453452No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1987970.4336108251834498No Hit
AGGAGGACGGACGGACGGACGGACGGGGCCCCCCGAGCCACCTTCCCCGC1846790.4028170122489491No Hit
TGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACGAGGA1484790.323858517545101No Hit
GGAGGGGTGGCCCGGCCCCCCCACGAGGAGACGCCGGCGCGCCCCCGCGG1384840.30205768454606896No Hit
CGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAGGGACGCA1369270.2986615968042487No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA1356960.2959765717495405No Hit
GGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACGAGGAG1216410.2653201727699111No Hit
GGACGGACGGACGGACGGACGGGGCCCCCCGAGCCACCTTCCCCGCCGGG1090140.23777849010069868No Hit
CCCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGTC1049620.22894037351119614No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT1027700.2241592403512283No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT981810.21414983338448912No Hit
GTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACGAGG950810.20738819433526456No Hit
GGAGGACGGACGGACGGACGGACGGGGCCCCCCGAGCCACCTTCCCCGCC925380.20184146914101359No Hit
GAGGAGGAGGACGGACGGACGGACGGACGGGGCCCCCCGAGCCACCTTCC909680.19841702613866435No Hit
GGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACGAGGAGA828870.18079095995905675No Hit
GGAGGAGGACGGACGGACGGACGGACGGGGCCCCCCGAGCCACCTTCCCC821190.17911581841395852No Hit
GGGAGGGGTGGCCCGGCCCCCCCACGAGGAGACGCCGGCGCGCCCCCGCG799200.1743194170367828No Hit
CGCCTCATTTGGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTT736460.16063473207070703No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGATATGGATATCGTA728530.15890506117843764TruSeq Adapter, Index 11 (97% over 38bp)
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT718530.15672388729159104No Hit
GGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAG692400.15102447992526077No Hit
GGGAGGAGGACGGACGGACGGACGGACGGGGCCCCCCGAGCCACCTTCCC687930.15004949519784033No Hit
TGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGAC650250.1418308319922022No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGATATGGATCTCGTAT639440.139472983020521TruSeq Adapter, Index 11 (97% over 38bp)
GTGCGATCGGCCCGAGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGC639150.13940972897780243No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC638370.1392395974146284No Hit
CGGGGGAGGAGGAGGACGGACGGACGGACGGACGGGGCCCCCCGAGCCAC605380.13204390476192138No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG592400.12921274105679445No Hit
GGGGTGGCCCGGCCCCCCCACGAGGAGACGCCGGCGCGCCCCCGCGGGGG565450.12333447743174278No Hit
AGGAGGAGGACGGACGGACGGACGGGGCCCCCCGAGCCACCTTCCCCGCC545440.11896994848416267No Hit
CCCGAGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCG544030.11866240296611728No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC543000.1184377420557721No Hit
GGACGGACGGACGGACGGGGCCCCCCGAGCCACCTTCCCCGCCGGGCCTT533020.11626093051669915No Hit
CCCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATC520120.113447216202667No Hit
CCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGC503310.10978066289887782No Hit
CCCCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGT493730.10769109831527875No Hit
GGAGGTTCTAGCAGGGGAGCGCAGCTACTCGTATACCCTTGACCGAAGAC491090.10711526840915123No Hit
CACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCAT491040.10710436253971699No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG475040.10361448432076238No Hit
CGGGGGACGGTCCCCCGCCGACCCCACCCCCGGCCCCGCCCGCCCACCCC465210.10147039038999216No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)