FastQCFastQC Report
Mon 6 Mar 2023
EGAF00000590826

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000590826
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences73631198
Sequences flagged as poor quality0
Sequence length90
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTACAAGATCTCGTAT5955670.8088514327853257TruSeq Adapter, Index 8 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTACAAGATCTCGTA5775890.7844351520669269TruSeq Adapter, Index 8 (97% over 36bp)
CCCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATC3426240.46532449465238906No Hit
TGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGA3303670.4486780182498185No Hit
GTCCCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAA2116490.2874447323266423No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1349430.18326878234413624No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC1227900.16676355041785412No Hit
CTCCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGG1214740.16497626454481973No Hit
GGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTACAAGATCTCGTA1108500.15054759804397044TruSeq Adapter, Index 8 (97% over 36bp)
AGAGCGGAAGAGCACACGTCTGAACTCCAGTCACACTACAAGATCTCGTA822980.111770556822938TruSeq Adapter, Index 8 (97% over 36bp)
GGGCGGAGGAGAGTAGTCTGAATTGGGTTATGAGGTCCCCTGCGGGGTAC821380.11155325762864812No Hit
GGGGGAGGAGGAGGACGGACGGACGGACGGACGGGGCCCCCCGAGCCACC819420.11128706611564299No Hit
GACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAG809630.10995746667058168No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)