FastQCFastQC Report
Fri 10 Mar 2023
EGAF00000590830

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000590830
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences75262500
Sequences flagged as poor quality0
Sequence length90
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATC3728260.49536754691911644No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCAGTAGATCTCGTA3547110.4712984554060788TruSeq Adapter, Index 6 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCAGTAGATCTCGTAT3097920.411615346287992TruSeq Adapter, Index 6 (97% over 37bp)
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC2078800.2762066101976416No Hit
GGGCGGAGGAGAGTAGTCTGAATTGGGTTATGAGGTCCCCTGCGGGGTAC1514900.20128217904002657No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC1312640.1744082378342468No Hit
GGGAGGGGTGGCCCGGCCCCCCCACGAGGAGACGCCGGCGCGCCCCCGCG1198710.15927055306427504No Hit
GGGGGTGCGTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCG1111100.1476299618003654No Hit
CGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAGGGACGCA966850.12846371034711843No Hit
GGCGGGGGTGCGTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACT942230.12519249294137186No Hit
GTCCCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAA939070.12477262913137353No Hit
GGGGTCTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTCA840120.11162531141006478No Hit
CGGGTCCTAACACGTGCGCTCGTGCTCCACCTCCCCGGCGCGGCGGGCGA836440.11113635608702872No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT826770.10985151968111609No Hit
GGAGGGGTCGGGAGGAACGGGGGGCGGGAAAGATCCGCCGGGCCGCCGAC792240.1052635774788241No Hit
GGGGGAGGAGGAGGACGGACGGACGGACGGACGGGGCCCCCCGAGCCACC779300.10354426175053978No Hit
CGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGT772290.10261285500747384No Hit
GGGCGCCTTAACCCGGCGTTCGGTTCATCCCGCAGCGCCAGTTCTGCTTA769580.10225278192991197No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)