FastQCFastQC Report
Sat 11 Mar 2023
EGAF00000590831

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000590831
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences75262500
Sequences flagged as poor quality0
Sequence length90
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC3743880.4974429496761335Illumina Single End PCR Primer 1 (100% over 50bp)
ACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACT3275340.43518883906327854No Hit
CGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGG1828250.24291645905995682No Hit
CAGGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTAT1756210.2333446271383491No Hit
AACCGTTAAGAGGTAAACGGGTGGGGTCCGCGCAGTCCGCCCGGAGGATT1152400.15311742235509052No Hit
AGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGT957040.12716027238000333No Hit
AAGATCAAGTGTAGTATCTGTTCTTATCAGTTTAATATCTGATACGTCCT936910.12448563361567846No Hit
AGGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATC912280.12121308752698888No Hit
CCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTC877580.11660255771466534No Hit
GGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATT866370.11511310413552565No Hit
CGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTGCATTTATCAGATCAAA834780.11091579471848531No Hit
GGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGC818140.10870486630127885No Hit
CGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCG756120.10046437468858994No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)