FastQCFastQC Report
Sat 25 May 2024
EGAF00000590833

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000590833
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences81295563
Total Bases7.3 Gbp
Sequences flagged as poor quality0
Sequence length90
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACT5538920.6813311570275982No Hit
CGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGG3124330.3843174073350104No Hit
CAGGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTAT2789160.34308883499582876No Hit
AGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGT2613660.32150094095541226No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC2455420.30203616401549493Illumina Single End PCR Primer 1 (100% over 50bp)
AAGATCAAGTGTAGTATCTGTTCTTATCAGTTTAATATCTGATACGTCCT2117000.26040781586075No Hit
GGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATT1721170.211717581684009No Hit
CAGCCCCCCCGGCAGCAGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAG1580130.1943685413679957No Hit
CGGGGCCGAGGGAGCGAGACCCGTCGCCGCGCTCTCCCCCCTCCCGGCGC1550050.1906684624350286No Hit
CCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTC1349300.16597461782754366No Hit
AGGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATC1306970.16076769158976117No Hit
AACCGTTAAGAGGTAAACGGGTGGGGTCCGCGCAGTCCGCCCGGAGGATT1070520.13168246340824283No Hit
CGACCATCCCCGATAGAGGAGGACCGGTCTTCGGTCAAGGGTATACGAGT985380.12120956711991772No Hit
CGACCGGCGACCGGCCGCCGCCGGGCGCATTTCCACCGCGGCGGTGCGCC900530.11077234313513519No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)