FastQCFastQC Report
Mon 6 Mar 2023
EGAF00000590834

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000590834
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences77605555
Sequences flagged as poor quality0
Sequence length90
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAAGATCTCGTAT6935680.8937092196557321TruSeq Adapter, Index 23 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAAGATCTCGTA6059820.7808487420778062TruSeq Adapter, Index 23 (97% over 38bp)
CCCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATC5011570.6457746484771612No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2356360.30363290359820244No Hit
GTCCCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAA2252580.29026014954728435No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC1207560.15560226326581905No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT1193260.1537596116669741No Hit
CTCCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGG1093190.14086491617771435No Hit
CCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCG949770.12238428035209593No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT933840.12033159224233368No Hit
AGAGCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAAGATCTCGTA849110.10941355937728942TruSeq Adapter, Index 7 (97% over 35bp)
GGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAAGATCTCGTA816100.10515999788932637TruSeq Adapter, Index 23 (97% over 38bp)
GGGCGGAGGAGAGTAGTCTGAATTGGGTTATGAGGTCCCCTGCGGGGTAC813660.10484558740672624No Hit
AGTCCCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAA786940.10140253490874462No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)