FastQCFastQC Report
Fri 10 Mar 2023
EGAF00000590836

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000590836
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences76180017
Sequences flagged as poor quality0
Sequence length90
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATC13894791.8239415724992554No Hit
GTCCCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAA5327110.699279182360907No Hit
CCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCG4581760.6014385636065164No Hit
CCCCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAAT3948100.5182592700130272No Hit
GCCCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAAT3114090.4087804285997993No Hit
TCCCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAAT2984960.3918297891689891No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC2602990.3416893435453027No Hit
CGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAGGGACGCA2327680.305549944941598No Hit
AGTCCCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAA2140800.28101857735211583No Hit
GGGCGGAGGAGAGTAGTCTGAATTGGGTTATGAGGTCCCCTGCGGGGTAC2064360.27098444989845566No Hit
GGTCGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCATCTCCTATTTC1979490.2598437330356595No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATAGGAAGATCTCGTA1826390.23974659916392507TruSeq Adapter, Index 3 (97% over 38bp)
GGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAA1519470.1994578184460106No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG1486110.1950787172966895No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT1314060.17249405444475027No Hit
ACCCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAAT1282710.16837880201575697No Hit
CCGCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATC1227260.16109999030323136No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATAGGAAGATCTCGTAT1153160.15137303001651997TruSeq Adapter, Index 3 (97% over 38bp)
AGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTG1148010.1506969997131925No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1049310.13774084613291698No Hit
CCCGCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATC996370.13079151715075096No Hit
GGTCCCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAA992750.13031632691812078No Hit
GGCCCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAA924210.12131921682296291No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT905190.11882249908135357No Hit
CGGTGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAGGGACGCA901470.11833418204671706No Hit
CGTGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAGGGACGCA871150.11435413567838924No Hit
AGGCCCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAA870130.11422024229792441No Hit
CTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGG856670.1124533747478686No Hit
CCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGC837840.10998159793007135No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT832850.10932657050995408No Hit
CACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCAT798750.10485033102578595No Hit
CCCCCGCTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATC797750.10471906300572235No Hit
AGGTCGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCATCTCCTATTT796260.1045234736558276No Hit
GGGCGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCATCTCCTATTTC774530.10167101957984598No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)