FastQCFastQC Report
Thu 23 Feb 2023
EGAF00000590838

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000590838
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences95187713
Sequences flagged as poor quality0
Sequence length90
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGAGCCCATGCTCGGGATATCTCAGGATATCTCAGGATATCTCAGGAT14514671.5248470146561879No Hit
CCTGACTGATATCCCGAGATATCCTGAGATATCCTGAGATATCCTGAGAT12724951.3368269495034513No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTTATATATCTCGTA12231761.2850145900658418TruSeq Adapter, Index 8 (97% over 37bp)
ATCCTGAGATATCCTGAGATATCCTGAGATATCCTGAGATATCCCGAGAT4532630.4761780546192974No Hit
ATCCTGAGATATCCTGAGATATCCTGAGATATCCCGAGATATCCTGAGAT3932640.4131457596843408No Hit
ATCCTGAGATATCCTGAGATATCCCGAGATATCCTGAGATATCCTGAGAT3882740.40790348645102964No Hit
ATCCTGAGATATCCCGAGATATCCTGAGATATCCTGAGATATCCTGAGAT3734310.39231008733238504No Hit
GGGCGGAGGAGAGTAGTCTGAATTGGGTTATGAGGTCCCCTGCGGGGTAC3076860.3232412990109343No Hit
CTGACTGATATCCCGAGATATCCTGAGATATCCTGAGATATCCTGAGATA2959880.3109518977517613No Hit
ATCCCGAGATATCCTGAGATATCCTGAGATATCCTGAGATATCCTGAGAT2798190.2939654616977718No Hit
CTCAGGATATCTCAGGATATCTCAGGATATCTCGGGATATCTCAGGATAT2765710.2905532565952078No Hit
CGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAGGGACGCA2748350.2887294917990098No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT2593400.27245113032603274No Hit
CTCAGGATATCTCAGGATATCTCGGGATATCTCAGGATATCTCAGGATAT2558690.2688046512893949No Hit
ATCTCAGGATATCTCAGGATATCTCAGGATATCTCGGGATATCTCAGGAT2556630.2685882368032101No Hit
ATCTCAGGATATCTCAGGATATCTCGGGATATCTCAGGATATCTCAGGAT2362720.24821691009636926No Hit
CTCAGGATATCTCGGGATATCTCAGGATATCTCAGGATATCTCAGGATAT2357680.24768742999424728No Hit
ATCTCGGGATATCTCAGGATATCTCAGGATATCTCAGGATATCTCAGGAT2333810.24517975339947498No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC2203110.23144898964008098No Hit
ATCTCAGGATATCTCGGGATATCTCAGGATATCTCAGGATATCTCAGGAT2147730.22563101185128798No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1923260.20204918674745342No Hit
CTCAGGATATCTCAGGATATCTCAGGATATCTCAGGATATCTCGGGATAT1839510.19325078227270784No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG1802120.18932275429287812No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTTATATATCTCGTAT1757840.18467089339566337TruSeq Adapter, Index 8 (97% over 37bp)
ATCTCAGGATATCTCAGGATATCTCAGGATATCTCAGGATATCTCGGGAT1670810.1755279066322352No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT1487850.1563069384805999No Hit
CTCGGGATATCTCAGGATATCTCAGGATATCTCAGGATATCTCAGGATAT1198810.12594167484620625No Hit
CACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCAT1134290.11916348909443805No Hit
GCGCCTCATTTGGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGT1124730.11815915778961934No Hit
CCGGAGCCCATGCTCGGGATATCTCAGGATATCTCAGGATATCTCAGGAT1100350.11559790285117996No Hit
GGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAA1094940.11502955218600536No Hit
GGAGGTTCTAGCAGGGGAGCGCAGCTACTCGTATACCCTTGACCGAAGAC1060600.1114219437124201No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG1019070.10705898564870447No Hit
GCCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATA1009680.10607251379177478No Hit
TCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAGGGACGC997790.10482340299530046No Hit
CTGAGCCCATGCTCGGGATATCTCAGGATATCTCAGGATATCTCAGGATA996030.10463850518186103No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)