FastQCFastQC Report
Mon 6 Feb 2023
EGAF00000590841

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000590841
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences133326302
Sequences flagged as poor quality0
Sequence length90
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGAGCCCATGCTCGGGATATCTCAGGATATCTCAGGATATCTCAGGAT21747261.6311305176678492No Hit
CCTGACTGATATCCCGAGATATCCTGAGATATCCTGAGATATCCTGAGAT17995291.3497179273749No Hit
CGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGG9416140.7062477439747785No Hit
ATCCTGAGATATCCTGAGATATCCTGAGATATCCTGAGATATCCCGAGAT6770320.5078007788740739No Hit
GGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATT6385140.4789107553586839No Hit
ATCCTGAGATATCCCGAGATATCCTGAGATATCCTGAGATATCCTGAGAT5751410.43137849874513134No Hit
ATCCTGAGATATCCTGAGATATCCTGAGATATCCCGAGATATCCTGAGAT5723280.42926863748159755No Hit
ATCCTGAGATATCCTGAGATATCCCGAGATATCCTGAGATATCCTGAGAT5712910.4284908464647883No Hit
AGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGT5349460.40123065889879705No Hit
ATCCCGAGATATCCTGAGATATCCTGAGATATCCTGAGATATCCTGAGAT4313940.32356256307176356No Hit
ACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACT4294660.3221164868129321No Hit
CTGACTGATATCCCGAGATATCCTGAGATATCCTGAGATATCCTGAGATA3860420.28954676924887635No Hit
ATCTCAGGATATCTCAGGATATCTCAGGATATCTCGGGATATCTCAGGAT3617420.2713208081028153No Hit
ATCTCGGGATATCTCAGGATATCTCAGGATATCTCAGGATATCTCAGGAT3599140.26994973579931736No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGG3434940.25763408633354284No Hit
CCTAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACGATCTCGT3407470.2555737276805292No Hit
ATCTCAGGATATCTCAGGATATCTCGGGATATCTCAGGATATCTCAGGAT3368630.2526605740553728No Hit
ATCTCAGGATATCTCGGGATATCTCAGGATATCTCAGGATATCTCAGGAT3229540.24222827390802454No Hit
CTCAGGATATCTCAGGATATCTCAGGATATCTCGGGATATCTCAGGATAT3174560.23810455644378406No Hit
CCTAAGCTTCTTACATGGCCCAGGTGCAGTACAGCATGTGGGTGACGCGA3172110.23792079675321676No Hit
CCTTCACGCTTCATACGCGGGTCATAGTTGGCAAAGTACCAGGCATTTTT3162330.23718725807005434No Hit
CTCAGGATATCTCAGGATATCTCGGGATATCTCAGGATATCTCAGGATAT3040490.2280487761522104No Hit
CTCAGGATATCTCGGGATATCTCAGGATATCTCAGGATATCTCAGGATAT2947590.2210809087017204No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGG2925530.2194263214470615No Hit
CCTGCATTATGTCGTGATTGAGCGGGATGAAAAGTACATGGCGAGTTTTG2762740.2072164275583073No Hit
ATCTCAGGATATCTCAGGATATCTCAGGATATCTCAGGATATCTCGGGAT2404100.18031700901747053No Hit
CGACCATCCCCGATAGAGGAGGACCGGTCTTCGGTCAAGGGTATACGAGT2337430.17531649531538046No Hit
CTCGGGATATCTCAGGATATCTCAGGATATCTCAGGATATCTCAGGATAT2240170.16802161061963602No Hit
CCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTT2160510.1620467955377627No Hit
AGGGAAGCTCATCAGTGGGGCCACGAGCTGAGTGCGTCCTGTCACTCCAC2152890.16147526539812077No Hit
CTCAGGATATCTCAGGATATCTCAGGATATCTCAGGATATCTCGGGATAT2142610.16070422473729154No Hit
TCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTG1767650.13258074164541067No Hit
CCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCA1740830.13056913556336394No Hit
CAGGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTAT1726190.12947107765728025No Hit
CCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCCCGAAGCTGCGCGCT1680110.12601489539550867No Hit
AACCGTTAAGAGGTAAACGGGTGGGGTCCGCGCAGTCCGCCCGGAGGATT1648050.12361026858751396No Hit
AAGATCAAGTGTAGTATCTGTTCTTATCAGTTTAATATCTGATACGTCCT1498410.11238667671139638No Hit
CCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTT1472850.11046957561306997No Hit
GAAGGCCTGTATCCTAGGCTACACACTGAGGACTCTGTTCCTCCCCTTTC1466230.10997304942876163No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1445750.10843696842352982Illumina Single End PCR Primer 1 (100% over 50bp)
GCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCG1368650.10265416346731043No Hit
AGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGT1351490.10136709559378614No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)