FastQCFastQC Report
Sat 25 May 2024
EGAF00000590845

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000590845
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences99415753
Total Bases8.9 Gbp
Sequences flagged as poor quality0
Sequence length90
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGAGCCCATGCTCGGGATATCTCAGGATATCTCAGGATATCTCAGGAT11672291.1740885772901606No Hit
CCTGACTGATATCCCGAGATATCCTGAGATATCCTGAGATATCCTGAGAT10187221.0247088306015244No Hit
CGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGG6329940.6367139823404043No Hit
GGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATT4334380.4359852306304012No Hit
AGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGT3052430.30703685360608796No Hit
ATCCTGAGATATCCTGAGATATCCTGAGATATCCTGAGATATCCCGAGAT2863480.2880308113745314No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGG2862780.28796039999817735No Hit
ATCCTGAGATATCCTGAGATATCCTGAGATATCCCGAGATATCCTGAGAT2460740.24752012892765596No Hit
ATCCTGAGATATCCTGAGATATCCCGAGATATCCTGAGATATCCTGAGAT2432190.24464835064921753No Hit
ATCCTGAGATATCCCGAGATATCCTGAGATATCCTGAGATATCCTGAGAT2398540.24126357520019992No Hit
CTGACTGATATCCCGAGATATCCTGAGATATCCTGAGATATCCTGAGATA2056380.20684649443836128No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGG2056050.20681330050379443No Hit
CCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCA2056000.20680827111976913No Hit
AAGATCAAGTGTAGTATCTGTTCTTATCAGTTTAATATCTGATACGTCCT2040120.2052109387533382No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1902210.1913388917347938Illumina Single End PCR Primer 1 (100% over 50bp)
ATCCCGAGATATCCTGAGATATCCTGAGATATCCTGAGATATCCTGAGAT1892150.1903269796689062No Hit
ACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACT1834560.18453413514858155No Hit
CAATATGGTGACCTCCCGGGAGCGGGGGACCACCAGGTTGCCTAAGGAGG1678310.1688173100695621No Hit
ATCTCAGGATATCTCAGGATATCTCAGGATATCTCGGGATATCTCAGGAT1625700.16352539219815596No Hit
CGTCCCGGCCCGGAGCGGACGAGCCGCCCCGGCGGTGAACGGGGAGGAGG1613730.16232135766250244No Hit
CCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCCCGAAGCTGCGCGCT1587710.15970406621574348No Hit
ATCTCGGGATATCTCAGGATATCTCAGGATATCTCAGGATATCTCAGGAT1522610.15315580821482086No Hit
ATCTCAGGATATCTCAGGATATCTCGGGATATCTCAGGATATCTCAGGAT1497570.1506370926949575No Hit
CTCAGGATATCTCAGGATATCTCAGGATATCTCGGGATATCTCAGGATAT1462330.14709238283393578No Hit
CATCAATATGGTGACCTCCCGGGAGCGGGGGACCACCAGGTTGCCTAAGG1432610.14410291696930566No Hit
ATCTCAGGATATCTCGGGATATCTCAGGATATCTCAGGATATCTCAGGAT1395230.14034294947200168No Hit
CTCAGGATATCTCAGGATATCTCGGGATATCTCAGGATATCTCAGGATAT1366960.13749933574410486No Hit
CCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTT1316680.13244178716827704No Hit
CTCAGGATATCTCGGGATATCTCAGGATATCTCAGGATATCTCAGGATAT1285930.12934871599272604No Hit
GGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGG1262770.12701910531221344No Hit
GTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGAC1226690.12338990179956691No Hit
CGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGA1181300.11882422698141208No Hit
TCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTG1174910.11818147170298052No Hit
CGACCATCCCCGATAGAGGAGGACCGGTCTTCGGTCAAGGGTATACGAGT1095440.11018776873319061No Hit
ATCTCAGGATATCTCAGGATATCTCAGGATATCTCAGGATATCTCGGGAT1063590.1069840511090833No Hit
GCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCACC1058770.1064992184890457No Hit
AAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCACCAGGTT1057400.10636141336675285No Hit
CCCGGGAGCGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCA1004470.10103730743758488No Hit
AGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGT1003720.10096186667720557No Hit
AGGGAAGCTCATCAGTGGGGCCACGAGCTGAGTGCGTCCTGTCACTCCAC995240.10010888314651704No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)