FastQCFastQC Report
Tue 13 Sep 2022
EGAF00000603731

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000603731
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10261446
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGGATGCATCTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTGAG263650.25693259994741485No Hit
ACTAGTATGGCCCGGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTT262040.2553636202928905No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC243760.23754936682412986Illumina Single End PCR Primer 1 (100% over 50bp)
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCCATCTG240540.23441140751508122No Hit
GGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTTCTAAGACCGTCTG231970.22605975804969397No Hit
AGTATGGCCCGGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTTCTA187460.18268380499200598No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTG184690.17998438036900452No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)