FastQCFastQC Report
Sat 1 Apr 2023
EGAF00000604875

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000604875
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31536045
Sequences flagged as poor quality0
Sequence length42
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATC23793307.544795170098216TruSeq Adapter, Index 8 (100% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGGATC936960.2971076430161106TruSeq Adapter, Index 8 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGCATC628190.19919745801986266TruSeq Adapter, Index 8 (97% over 42bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)