FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000605195

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000605195
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7577971
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTAGTATGGCCCGGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAA1880812.4819440454443544No Hit
GGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAACCCTTTAAGAGTT1817912.398940296815599No Hit
ACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAATCTA1708172.254125807554555No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACGT1579602.0844629782827093No Hit
AGTATGGCCCGGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAACCC789141.041360543607253No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAATC754340.9954379609000878No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTAT283480.3740843030410119TruSeq Adapter, Index 22 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTATG96680.12758032460140056TruSeq Adapter, Index 22 (97% over 40bp)
AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTT94620.12486191884344766Illumina Single End PCR Primer 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)