FastQCFastQC Report
Mon 12 Sep 2022
EGAF00000605204

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000605204
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6364244
Sequences flagged as poor quality0
Sequence length51
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC1304022.0489786375255252TruSeq Adapter, Index 9 (100% over 51bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC147670.23203070152558575TruSeq Adapter, Index 9 (100% over 50bp)
ACTAGTATGGCCCGGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAA101260.15910766463385126No Hit
ACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAATCTA97630.15340392354535748No Hit
GGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAACCCTTTAAGAGTT96440.15153410208659504No Hit
TCTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTGAGGGTTATAAGCT91590.1439134011832356No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACGT87710.13781684046054804No Hit
GATCCTTATCTGTCAAAACCGCTAATGTCCGTTCTAAGACCGTCTGGAGAA85330.13407719754302316No Hit
TCTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAATCTAAAACTTCGAT81210.1276035299715096No Hit
ACTGGATGCATCTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTGAGG80860.12705358248363827No Hit
GGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTTCTAAGACCGTCTGG80610.1266607628494445No Hit
ACTAGTATGGCCCGGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTTC79400.12475951581994657No Hit
GATCCTACGTTCCAAATGCAGCGAGCTCGTATAACCCTTTAAGAGTTGCTC79240.12450811125406254No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCCATCTGC77720.12211976787816431No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)