FastQCFastQC Report
Tue 13 Sep 2022
EGAF00000605210

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000605210
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12009048
Sequences flagged as poor quality0
Sequence length51
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTTCTAAGACCGTCTGG178980.14903762563027478No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCCATCTGC175780.14637296811537434No Hit
GGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAACCCTTTAAGAGTT174680.1454569920946273No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATG166440.1385954989937587TruSeq Adapter, Index 7 (97% over 36bp)
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACGT155880.12980212919458728No Hit
ACTGGATGCATCTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTGAGG128860.10730242730314675No Hit
ACTAGTATGGCCCGGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTTC123420.10277250952781602No Hit
TCTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTGAGGGTTATAAGCT120380.10024108488866063No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)