FastQCFastQC Report
Sun 2 Apr 2023
EGAF00000605263

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000605263
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44880790
Sequences flagged as poor quality0
Sequence length51
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC7694301.7143860435611762TruSeq Adapter, Index 2 (100% over 51bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC2097910.46744052410842146TruSeq Adapter, Index 2 (100% over 50bp)
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCCATCTGC737220.16426181446449584No Hit
GGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTTCTAAGACCGTCTGG733590.16345300517214603No Hit
ACTGGATGCATCTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTGAGG716360.15961394618944988No Hit
GGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAACCCTTTAAGAGTT705920.1572877839271546No Hit
ACTAGTATGGCCCGGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTTC694340.15470761544081554No Hit
ACTAGTATGGCCCGGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAA664780.14812127861385685No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACGT650620.14496625393626092No Hit
ACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAATCTA646920.14414184777050493No Hit
TCTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTGAGGGTTATAAGCT454500.10126827090164857No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)