FastQCFastQC Report
Sun 2 Apr 2023
EGAF00000605277

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000605277
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38509865
Sequences flagged as poor quality0
Sequence length51
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC11928493.097515402871446TruSeq Adapter, Index 9 (100% over 51bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC1355150.3518968451330588TruSeq Adapter, Index 9 (100% over 50bp)
ACTAGTATGGCCCGGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAA645510.16762198465250397No Hit
TCTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTGAGGGTTATAAGCT621280.16133009035477014No Hit
ACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAATCTA613270.1592501038370298No Hit
GGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAACCCTTTAAGAGTT610980.15865545101235748No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACGT571510.14840612918274318No Hit
GATCCTTATCTGTCAAAACCGCTAATGTCCGTTCTAAGACCGTCTGGAGAA568090.14751804505157315No Hit
ACTAGTATGGCCCGGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTTC556550.14452141029318072No Hit
ACTGGATGCATCTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTGAGG546890.14201296213320924No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCCATCTGC534280.13873847649167298No Hit
GGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTTCTAAGACCGTCTGG531340.13797503574733383No Hit
TCTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAATCTAAAACTTCGAT515400.13383583660965834No Hit
GATCCTACGTTCCAAATGCAGCGAGCTCGTATAACCCTTTAAGAGTTGCTC493890.1282502548372995No Hit
GATCGGAAGAGCACACGTCGGAACTCCAGTCACGATCAGATCTCGTATGCC421780.10952518270318527TruSeq Adapter, Index 9 (98% over 51bp)
GTACTGGATGCATCTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTGA403630.10481210463864259No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAATC396790.10303593637630254No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)