FastQCFastQC Report
Mon 18 Sep 2023
EGAF00000605281

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000605281
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75652424
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC32252804.2632870560763525TruSeq Adapter, Index 9 (100% over 51bp)
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCCATCTGC15377552.0326579357192838No Hit
GGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTTCTAAGACCGTCTGG15288542.0208922849583777No Hit
GGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAACCCTTTAAGAGTT13049061.7248700451422416No Hit
ACTAGTATGGCCCGGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTTC12544211.6581372197670758No Hit
ACTGGATGCATCTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTGAGG12402081.6393499830223548No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACGT12160131.6073681921943441No Hit
ACTAGTATGGCCCGGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAA10775681.4243667856564648No Hit
ACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAATCTA10200621.348353358776713No Hit
GGGGGATCCTAGAGACCATTCGCGATTCCATGAGACTCCAAGGGTTCTGCA7538430.9964558438999918No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC7316440.9671124351547546TruSeq Adapter, Index 9 (100% over 50bp)
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCGTCTTCA7252740.9586923480469045No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTGA6427060.8495510996448706No Hit
ACTAGTATGGCCCGGGGGATCCTAGAGACCATTCGCGATTCCATGAGACTC6219140.8220675123377408No Hit
ACTGGATGCATCTGCAGGATATCGCGGCCGCGTCTTCAGAGGGGGATAGCA5948150.786247113509542No Hit
AGTATGGCCCGGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTTCTAA5863490.7750564608478375No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCAGAAGAC5791310.7655154579052219No Hit
GGGGGATCCGTTAGCTATCGTTCGCGAGAAAGTTAGTAGACACACAGGACC5408350.7148944758201006No Hit
GGGGGATCCGTATACGTTTCTAATTTGTAGTTAACGGTTGGATACCACTTT5330820.7046462913072025No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAATC5042650.6665549804458348No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCTCTAAAC4965740.6563887496850068No Hit
AGTATGGCCCGGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAACCC4747880.6275912586753334No Hit
ACTGGATGCATCTGCAGGATATCGCGGCCGCAGAAGACCTAAGCTAGCGTT4521690.5976926793515566No Hit
GGGGGATCCGCTCGCACTTAGCCTGTTAAGGGGTTCGCGCTCGTCTAGTCT4371120.5777898141109133No Hit
ACTAGTATGGCCCGGGGGATCCGTATACGTTTCTAATTTGTAGTTAACGGT4146540.5481040501755766No Hit
ACTAGTATGGCCCGGGGGATCCGTTAGCTATCGTTCGCGAGAAAGTTAGTA4024250.5319393334970999No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCACGCCCA3948320.5219026425379311No Hit
ACTGGATGCATCTGCAGGATATCGCGGCCGCTCTAAACGTGGTAAATTGTT3692950.48814694952801513No Hit
GGGGGATCCTTGGACCGTTAATTCATATATCGAAGTAGCAGGTTGTTGCCC3537860.4676466149980865No Hit
ACTAGTATGGCCCGGGGGATCCGCTCGCACTTAGCCTGTTAAGGGGTTCGC3031440.40070626157332384No Hit
ACTGGATGCATCTGCAGGATATCGCGGCCGCACGCCCATATAAACGATCGG2928590.38711119157265866No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCGTCTTCAGAGGGGGATAG2922100.3862533208453439No Hit
AGTATGGCCCGGGGGATCCTAGAGACCATTCGCGATTCCATGAGACTCCAA2735100.3615350117532255No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCGAGTCAC2673510.3533938317693561No Hit
GGGGGATCCTTAAGTCGTGTCCTTCTCCTACGATCTTGTGAACGATGGATA2660960.3517349292072915No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCACGGTCA2243260.29652189333682155No Hit
ACTAGTATGGCCCGGGGGATCCTTGGACCGTTAATTCATATATCGAAGTAG2189800.28945536497283947No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCAGAAGACCTAAGCTAGCG2167150.28646140935285824No Hit
ACTGGATGCATCTGCAGGATATCGCGGCCGCGAGTCACTGTTAGAAGTCCC1899140.2510349172684804No Hit
AGTATGGCCCGGGGGATCCGTATACGTTTCTAATTTGTAGTTAACGGTTGG1808920.23910932450756633No Hit
AGTATGGCCCGGGGGATCCGTTAGCTATCGTTCGCGAGAAAGTTAGTAGAC1581530.20905212501849246No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCTCTAAACGTGGTAAATTG1578410.20863971258872024No Hit
ACTGGATGCATCTGCAGGATATCGCGGCCGCACGGTCATCAATTCTTTAAG1549900.20487116182820528No Hit
ACTAGTATGGCCCGGGGGATCCTTAAGTCGTGTCCTTCTCCTACGATCTTG1420280.18773754030670584No Hit
GGGGGATCCGGCGCGCCTAGGCCTTGACGGCCTTCACTCAATTCGCCCTAT1405690.18580898346363628No Hit
GGGCTCGCGCTGGTTTCCAGCAGATCGTTAATCAGCGCACGACGGGTGGTT1056540.13965712453575843No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCACGCCCATATAAACGATC921630.12182425245224132No Hit
AGTATGGCCCGGGGGATCCTTGGACCGTTAATTCATATATCGAAGTAGCAG845860.11180871084844551No Hit
AGTATGGCCCGGGGGATCCGCTCGCACTTAGCCTGTTAAGGGGTTCGCGCT771820.1020218466496196No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCGAGTCACTGTTAGAAGTC769770.10175087053390383No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)