FastQCFastQC Report
Sat 1 Apr 2023
EGAF00000605283

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000605283
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27488180
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATG1644190.5981443660511536TruSeq Adapter, Index 14 (97% over 44bp)
ACTAGTATGGCCCGGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAA1058710.38515099944776265No Hit
GGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAACCCTTTAAGAGTT1036910.37722031796939626No Hit
ACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAATCTA1019960.3710540312236023No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACGT960420.34939381217672466No Hit
ACTGGATGCATCTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTGAGG941930.3426672846292479No Hit
ACTAGTATGGCCCGGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTTC911430.3315716064141023No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCCATCTGC874100.3179912238642209No Hit
GGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTTCTAAGACCGTCTGG867990.31576845029390815No Hit
TCTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTGAGGGTTATAAGCT858350.3122614883924654No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT856330.31152662708116724TruSeq Adapter, Index 14 (97% over 44bp)
GATCCTTATCTGTCAAAACCGCTAATGTCCGTTCTAAGACCGTCTGGAGAA728080.26487020966830105No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTGA587240.21363364180531416No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAATC583060.21211298820074664No Hit
AGTATGGCCCGGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAACCC554570.2017485333696156No Hit
TCTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAATCTAAAACTTCGAT548540.19955486321757207No Hit
GATCCTACGTTCCAAATGCAGCGAGCTCGTATAACCCTTTAAGAGTTGCTC523850.19057282075422963No Hit
AGTATGGCCCGGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTTCTAA521720.1897979422428113No Hit
ACTAGTATGGCCCGGGGGATCCTAGAGACCATTCGCGATTCCATGAGACTC439860.16001786949881733No Hit
ACTGGATGCATCTGCAGGATATCGCGGCCGCGTCTTCAGAGGGGGATAGCA435870.15856633651263924No Hit
GGGGGATCCTAGAGACCATTCGCGATTCCATGAGACTCCAAGGGTTCTGCA413850.15055562063403252No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCGTCTTCA398120.14483316101684432No Hit
TCTGCAGGATATCGCGGCCGCGTCTTCAGAGGGGGATAGCATGACCTCACG395460.1438654723593923No Hit
GATCCTAGAGACCATTCGCGATTCCATGAGACTCCAAGGGTTCTGCACAAC382150.13902339114484843No Hit
ACTGGATGCATCTGCAGGATATCGCGGCCGCAGAAGACCTAAGCTAGCGTT333590.12135761625542325No Hit
TCTGCAGGATATCGCGGCCGCAGAAGACCTAAGCTAGCGTTTAGAGTGCAA315580.11480570921756188No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCAGAAGAC310710.11303403863042225No Hit
GATCCGTATACGTTTCTAATTTGTAGTTAACGGTTGGATACCACTTTGAGG300380.10927605974640736No Hit
ACTAGTATGGCCCGGGGGATCCGTATACGTTTCTAATTTGTAGTTAACGGT291980.10622020082813777No Hit
GGGGGATCCGTTAGCTATCGTTCGCGAGAAAGTTAGTAGACACACAGGACC291810.10615835606431565No Hit
ACTAGTATGGCCCGGGGGATCCGTTAGCTATCGTTCGCGAGAAAGTTAGTA285040.1036954792932817No Hit
GGGGGATCCGTATACGTTTCTAATTTGTAGTTAACGGTTGGATACCACTTT284880.10363727245674323No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCGTCTTCAGAGGGGGATAG276610.10062870659316113No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)