FastQCFastQC Report
Sun 11 Sep 2022
EGAF00000605365

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000605365
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4180541
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCC1501093.5906596777785458TruSeq Adapter, Index 11 (100% over 51bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC341080.8158752659045803TruSeq Adapter, Index 11 (100% over 50bp)
ACTAGTATGGCCCGGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAA226400.5415567028286531No Hit
ACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAATCTA221470.5297639707396722No Hit
ACTGGATGCATCTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTGAGG192260.45989263112118745No Hit
ACTAGTATGGCCCGGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTTC188820.4516640310428722No Hit
GGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAACCCTTTAAGAGTT185290.44322014782297314No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACGT177550.42470579764676386No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCCATCTGC153660.36756008373078985No Hit
GGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTTCTAAGACCGTCTGG143120.34234803581641704No Hit
ACTAGTATGGCCCGGGGGATCCTAGAGACCATTCGCGATTCCATGAGACTC99940.2390599685543091No Hit
ACTGGATGCATCTGCAGGATATCGCGGCCGCGTCTTCAGAGGGGGATAGCA99880.2389164464599199No Hit
GGGGGATCCTAGAGACCATTCGCGATTCCATGAGACTCCAAGGGTTCTGCA80870.19344386288760235No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCGTCTTCA79210.18947308494283396No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAATC71610.17129361965353287No Hit
ACTAGTATGGCCCGGGGGATCCGTATACGTTTCTAATTTGTAGTTAACGGT68100.16289757713176356No Hit
AGTATGGCCCGGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAACCC67810.16220388700888233No Hit
ACTGGATGCATCTGCAGGATATCGCGGCCGCAGAAGACCTAAGCTAGCGTT65710.15718061370525968No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTGA64990.15545834857258906No Hit
ACTAGTATGGCCCGGGGGATCCGTTAGCTATCGTTCGCGAGAAAGTTAGTA63230.15124836713717196No Hit
ACTGGATGCATCTGCAGGATATCGCGGCCGCTCTAAACGTGGTAAATTGTT61570.14727758919240358No Hit
GGGGGATCCGTTAGCTATCGTTCGCGAGAAAGTTAGTAGACACACAGGACC59470.1422543158887809No Hit
GGGGGATCCGTATACGTTTCTAATTTGTAGTTAACGGTTGGATACCACTTT59450.14220647519065116No Hit
AGTATGGCCCGGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTTCTAA59240.1417041478602889No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCAGAAGAC55740.13333202568758445No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCTCTAAAC53620.12826091168583204No Hit
ACTGGATGCATCTGCAGGATATCGCGGCCGCACGCCCATATAAACGATCGG46590.11144490629322855No Hit
ACTAGTATGGCCCGGGGGATCCGCTCGCACTTAGCCTGTTAAGGGGTTCGC46560.11137314524603395No Hit
GGGGGATCCGCTCGCACTTAGCCTGTTAAGGGGTTCGCGCTCGTCTAGTCT45160.10802429637695218No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCACGCCCA42560.10180500562008601No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)