FastQCFastQC Report
Tue 13 Sep 2022
EGAF00000605382

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000605382
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15063170
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCC155374110.314834128540008TruSeq Adapter, Index 8 (100% over 51bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC3807942.52798049812888TruSeq Adapter, Index 8 (100% over 50bp)
ACTAGTATGGCCCGGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAA1670871.1092419457524545No Hit
GGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAACCCTTTAAGAGTT1613691.07128180854362No Hit
ACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAATCTA1605501.0658447059948204No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACGT1515931.0063817908182675No Hit
ACTGGATGCATCTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTGAGG1404350.9323070774611187No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCCATCTGC1347860.894805011162989No Hit
ACTAGTATGGCCCGGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTTC1236000.8205444139580181No Hit
GGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTTCTAAGACCGTCTGG1099080.72964721237296No Hit
ACTGGATGCATCTGCAGGATATCGCGGCCGCGTCTTCAGAGGGGGATAGCA954540.6336913146435976No Hit
ACTAGTATGGCCCGGGGGATCCTAGAGACCATTCGCGATTCCATGAGACTC929680.6171874844405261No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCGTCTTCA916210.6082451436185079No Hit
GGGGGATCCTAGAGACCATTCGCGATTCCATGAGACTCCAAGGGTTCTGCA896240.5949876420434742No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCAGAAGAC717790.4765198826010727No Hit
ACTGGATGCATCTGCAGGATATCGCGGCCGCAGAAGACCTAAGCTAGCGTT717230.47614811490542824No Hit
GGGGGATCCGTTAGCTATCGTTCGCGAGAAAGTTAGTAGACACACAGGACC709270.47086370266019706No Hit
GGGGGATCCGTATACGTTTCTAATTTGTAGTTAACGGTTGGATACCACTTT686480.4557340851892397No Hit
ACTAGTATGGCCCGGGGGATCCGTATACGTTTCTAATTTGTAGTTAACGGT643350.4271013339157694No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCTCTAAAC616320.4091569038920759No Hit
ACTAGTATGGCCCGGGGGATCCGTTAGCTATCGTTCGCGAGAAAGTTAGTA598360.39723378279605154No Hit
ACTGGATGCATCTGCAGGATATCGCGGCCGCTCTAAACGTGGTAAATTGTT578450.3840161134741226No Hit
GGGGGATCCGCTCGCACTTAGCCTGTTAAGGGGTTCGCGCTCGTCTAGTCT522000.346540602011396No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCACGCCCA491350.3261929593837154No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAATC474380.3149270704639196No Hit
AGTATGGCCCGGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAACCC454870.30197494949602244No Hit
ACTAGTATGGCCCGGGGGATCCGCTCGCACTTAGCCTGTTAAGGGGTTCGC446320.29629885342859436No Hit
ACTGGATGCATCTGCAGGATATCGCGGCCGCACGCCCATATAAACGATCGG445220.29556859545500713No Hit
GGGGGATCCTTGGACCGTTAATTCATATATCGAAGTAGCAGGTTGTTGCCC425130.28223142937376394No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTGA393680.26135269003801986No Hit
AGTATGGCCCGGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTTCTAA338410.2246605462196868No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCGAGTCAC322920.21437718620980845No Hit
ACTAGTATGGCCCGGGGGATCCTTGGACCGTTAATTCATATATCGAAGTAG319600.21217313487134512No Hit
GGGGGATCCTTAAGTCGTGTCCTTCTCCTACGATCTTGTGAACGATGGATA310230.20595266467815207No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCACGGTCA283680.1883268926792966No Hit
ACTGGATGCATCTGCAGGATATCGCGGCCGCGAGTCACTGTTAGAAGTCCC282470.18752360890835063No Hit
ACTGGATGCATCTGCAGGATATCGCGGCCGCACGGTCATCAATTCTTTAAG238460.1583066512560105No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCGTCTTCAGAGGGGGATAG224600.14910540078881138No Hit
ACTAGTATGGCCCGGGGGATCCTTAAGTCGTGTCCTTCTCCTACGATCTTG210240.1395722148790726No Hit
AGTATGGCCCGGGGGATCCTAGAGACCATTCGCGATTCCATGAGACTCCAA207050.13745446675566964No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCAGAAGACCTAAGCTAGCG163750.10870885743173582No Hit
GGGGGATCCGGCGCGCCTAGGCCTTGACGGCCTTCACTCAATTCGCCCTAT150800.10011172946995885No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)