FastQCFastQC Report
Thu 5 Jan 2023
EGAF00000605474

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000605474
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40579889
Sequences flagged as poor quality0
Sequence length42
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAA6082821.4989740361290786No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACA3015430.7430848319964601No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATC830110.2045619198219098TruSeq Adapter, Index 2 (100% over 42bp)
GTGGGGGGGAGGGCGCGCGACCCCGGTCGGCGCGCCCCGCTT818380.2016713254193475No Hit
GGGGTGGGGGGGAGGGCGCGCGACCCCGGTCGGCGCGCCCCG715270.17626218741012328No Hit
GTGGGGGTGGGGGGGAGGGCGCGCGACCCCGGTCGGCGCGCC677530.16696201411492279No Hit
GGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATG545730.13448287155245792No Hit
GGGGGTGGGGGGGAGGGCGCGCGACCCCGGTCGGCGCGCCCC540390.13316694878095894No Hit
GTGGTGGTGGGGGTGGGGGGGAGGGCGCGCGACCCCGGTCGG507790.12513341276019754No Hit
CTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCT491730.1211757873462887No Hit
GTGGTGGGGGTGGGGGGGAGGGCGCGCGACCCCGGTCGGCGC450040.11090222548415546No Hit
GGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCT423680.1044063969716625No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)