FastQCFastQC Report
Sat 1 Apr 2023
EGAF00000605475

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000605475
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35595950
Sequences flagged as poor quality0
Sequence length42
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATC4502401.26486299705444TruSeq Adapter, Index 4 (100% over 42bp)
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAA2089930.5871257825679607No Hit
GTGGGGGGGAGGGCGCGCGACCCCGGTCGGCGCGCCCCGCTT1328550.3732306624770515No Hit
GGGGTGGGGGGGAGGGCGCGCGACCCCGGTCGGCGCGCCCCG1051410.2953734905235006No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACA996660.2799925272397562No Hit
GTGGGGGTGGGGGGGAGGGCGCGCGACCCCGGTCGGCGCGCC957790.2690727456353883No Hit
GGGGGTGGGGGGGAGGGCGCGCGACCCCGGTCGGCGCGCCCC732910.20589701918336217No Hit
GTGGTGGTGGGGGTGGGGGGGAGGGCGCGCGACCCCGGTCGG600870.16880291156718671No Hit
GTGGTGGGGGTGGGGGGGAGGGCGCGCGACCCCGGTCGGCGC581020.16322643446796617No Hit
GGGGGGAGGGCGCGCGACCCCGGTCGGCGCGCCCCGCTTCTT553190.15540812929560807No Hit
GGGGGGGAGGGCGCGCGACCCCGGTCGGCGCGCCCCGCTTCT364630.10243581081555626No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)