FastQCFastQC Report
Sun 2 Apr 2023
EGAF00000605476

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000605476
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38739694
Sequences flagged as poor quality0
Sequence length42
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATC12542293.237581071239231TruSeq Adapter, Index 5 (100% over 42bp)
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAA2522990.6512674054678904No Hit
GTGGGGGGGAGGGCGCGCGACCCCGGTCGGCGCGCCCCGCTT1988510.5133003890015239No Hit
GGGGTGGGGGGGAGGGCGCGCGACCCCGGTCGGCGCGCCCCG1468040.3789498182406913No Hit
GTGGGGGTGGGGGGGAGGGCGCGCGACCCCGGTCGGCGCGCC1237600.31946561064731177No Hit
GGGGGTGGGGGGGAGGGCGCGCGACCCCGGTCGGCGCGCCCC1084450.2799325157292156No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACA1080680.2789593536799749No Hit
GGGGGGAGGGCGCGCGACCCCGGTCGGCGCGCCCCGCTTCTT798720.20617612519087009No Hit
GTGGTGGGGGTGGGGGGGAGGGCGCGCGACCCCGGTCGGCGC724090.186911646746616No Hit
GTGGTGGTGGGGGTGGGGGGGAGGGCGCGCGACCCCGGTCGG684560.17670764255391383No Hit
GGGGGGGAGGGCGCGCGACCCCGGTCGGCGCGCCCCGCTTCT557590.14393247401489542No Hit
GGTGGGGGGGAGGGCGCGCGACCCCGGTCGGCGCGCCCCGCT473610.12225445043525641No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCAC455840.11766742401217727No Hit
GGTGGGGGTGGGGGGGAGGGCGCGCGACCCCGGTCGGCGCGC448950.11588888647390969No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)