FastQCFastQC Report
Tue 26 Sep 2023
EGAF00000605490

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000605490
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64262390
Sequences flagged as poor quality0
Sequence length42
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATC2624660.40842863142811836TruSeq Adapter, Index 2 (100% over 42bp)
CGGGGAGCGTGGTTTGGGAGCCGCGGAGGCGGCCGCGCCGAG924500.14386330791618548No Hit
GTCCGGGGAGCGTGGTTTGGGAGCCGCGGAGGCGGCCGCGCC758640.11805349909955107No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAA742510.11554347729675164No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)