FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000605492

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000605492
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23756721
Sequences flagged as poor quality0
Sequence length42
%GC58

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATC6143962.586198659318346TruSeq Adapter, Index 5 (100% over 42bp)
CGGGGAGCGTGGTTTGGGAGCCGCGGAGGCGGCCGCGCCGAG3472271.4615948051079943No Hit
GTCCGGGGAGCGTGGTTTGGGAGCCGCGGAGGCGGCCGCGCC1911420.8045807331744141No Hit
GGGGAGCGTGGTTTGGGAGCCGCGGAGGCGGCCGCGCCGAGC1547650.6514577495774774No Hit
CCGGGGAGCGTGGTTTGGGAGCCGCGGAGGCGGCCGCGCCGA1394180.5868570835175444No Hit
GTGGTTTGGGAGCCGCGGAGGCGGCCGCGCCGAGCCGGGCCC1314810.5534475906839164No Hit
GCGTGGTTTGGGAGCCGCGGAGGCGGCCGCGCCGAGCCGGGC1040210.4378592483365023No Hit
AGCGTGGTTTGGGAGCCGCGGAGGCGGCCGCGCCGAGCCGGG1011840.42591736460599927No Hit
GGGAGCGTGGTTTGGGAGCCGCGGAGGCGGCCGCGCCGAGCC997510.41988538738153297No Hit
GGTCCGGGGAGCGTGGTTTGGGAGCCGCGGAGGCGGCCGCGC798770.33622906124123775No Hit
GTTTGGGAGCCGCGGAGGCGGCCGCGCCGAGCCGGGCCCGTG784360.33016340933582544No Hit
GGTTTGGGAGCCGCGGAGGCGGCCGCGCCGAGCCGGGCCCGT771950.32493962445406505No Hit
CGTGGTTTGGGAGCCGCGGAGGCGGCCGCGCCGAGCCGGGCC709270.2985555119328126No Hit
GGAGCGTGGTTTGGGAGCCGCGGAGGCGGCCGCGCCGAGCCG688290.28972432685470356No Hit
CGGGGAGCGTGGTTTGGGAGCCGCGGAGGCGGCCGCGCCCAG541720.22802810202636972No Hit
GTCCGCTCCGGGCCGGGACGGGGTCCGGGGAGCGTGGTTTGG465340.1958772003931014No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAA418330.1760891160021621No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAAC388300.1634484826420279No Hit
GGGGGAGCGTGGTTTGGGAGCCGCGGAGGCGGCCGCGCCGAG334160.14065914231176938No Hit
GCCGCGCCGAGCCGGGCCCGTGGCCCGCCGGTCCCCGTCCCG300180.1263558215799226No Hit
GGGTCCGGGGAGCGTGGTTTGGGAGCCGCGGAGGCGGCCGCG291560.12272737470798264No Hit
GCTGGCAGGTCCCAGAAGCAGGAGATGGCCGAGAAGATGGTC289030.12166241292306292No Hit
CCGCTCCGGGCCGGGACGGGGTCCGGGGAGCGTGGTTTGGGA268320.11294487989314686No Hit
ACGGGGTCCGGGGAGCGTGGTTTGGGAGCCGCGGAGGCGGCC262980.11069709493999613No Hit
GCGGGGGGCCCGGCGGGGCGTGCGCGTCCGGCGCCGTCCGTC255970.10774635102209602No Hit
CTCCGGGCCGGGACGGGGTCCGGGGAGCGTGGTTTGGGAGCC255610.10759481495783868No Hit
GGGACGGGGTCCGGGGAGCGTGGTTTGGGAGCCGCGGAGGCG247780.10429890556024124No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTC241210.10153337238754456No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)