FastQCFastQC Report
Sun 2 Apr 2023
EGAF00000605493

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000605493
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51575373
Sequences flagged as poor quality0
Sequence length42
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATC3531410.6847085720543408TruSeq Adapter, Index 6 (100% over 42bp)
GTGCTGGCAGGTCCCAGAAGCAGGAGATGGCCGAGAAGATGG1978190.3835532124993066No Hit
GTCCGCTCCGGGCCGGGACGGGGTCCGGGGAGCGTGGTTTGG1271870.24660413023091465No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1239100.2402503225715886No Hit
GCTGGCAGGTCCCAGAAGCAGGAGATGGCCGAGAAGATGGTC1211830.23496291534333644No Hit
CCCGCCTCCTCCCCGTTCACCGCCGGGGCGGCTCGTCCGCTC1082160.20982107099836192No Hit
CGGGGAGCGTGGTTTGGGAGCCGCGGAGGCGGCCGCGCCGAG990280.19200636706980287No Hit
GTCCGGGGAGCGTGGTTTGGGAGCCGCGGAGGCGGCCGCGCC964090.18692836210801617No Hit
CCGGGGAGCGTGGTTTGGGAGCCGCGGAGGCGGCCGCGCCGA758170.1470023299686073No Hit
CCGCTCCGGGCCGGGACGGGGTCCGGGGAGCGTGGTTTGGGA700330.13578767525345867No Hit
GGGGAGCGTGGTTTGGGAGCCGCGGAGGCGGCCGCGCCGAGC650740.12617262118492095No Hit
GGTCCGGGGAGCGTGGTTTGGGAGCCGCGGAGGCGGCCGCGC581200.11268944191639681No Hit
CTCCGGGCCGGGACGGGGTCCGGGGAGCGTGGTTTGGGAGCC546820.10602346976724725No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)