FastQCFastQC Report
Tue 26 Sep 2023
EGAF00000639888

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000639888
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53170086
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTA1874820.35260804355291053TruSeq Adapter, Index 19 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT988860.18598051543493835TruSeq Adapter, Index 19 (97% over 40bp)
CTTGGATTAAGGCGACAGCGATTTCTAGGATAGTCAGTAGAATTAGAATT690200.12980983329611318No Hit
CCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGTGGCCTTGGTATG534260.10048131199185949No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)